A Critical Study on the Potential of Solid-Phase Fluorescence Spectroscopy (SPFS) in Quantitative Analysis: Effect of Solid Diluent and Sampling Method on Signal Quality

Basit Öğe Kaydı
dc.authoridGezici, Orhan/0000-0001-6808-2282
dc.authoridOzturk, Merve/0000-0002-8567-6418
dc.contributor.authorOzturk, Merve
dc.contributor.authorGezici, Orhan
dc.date.accessioned2024-11-07T13:24:27Z
dc.date.available2024-11-07T13:24:27Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground Solid-phase fluorescence spectroscopy (SPFS) is a very useful non-destructive technique for directly analyzing samples in solid form without the use of solvents. However, due to the so-called inner-filter effect, it is sometimes necessary to dilute solid samples using non-fluorescent solids as diluents. Objective This study aimed to explore the potential of SPFS in the quantitative analysis of fluorescent species based on: (1) the type of solid diluent; and (2) the sampling method used in the SPFS analysis. Methods Four different solids were used as solid diluents in the preparation of standard mixtures having different concentrations of rhodamine b and fluorescein as model compounds. Standard mixtures of model compounds were sampled by two different methods called: (1) the powder-cell method; and (2) the adhesive tape method. LOQ and calibration sensitivity calculated from the calibration graphs were used to assess the measurement performance. The usability of SPFS in real-sample analyses was also evaluated in detail. Results Among the solid diluents studied, the best results were obtained with sodium carbonate. The powder-cell method yielded a significant advantage over the adhesive tape method. The lowest LOQs for rhodamine b and fluorescein were obtained by sodium carbonate and the powder-cell method as 0.06 mg/kg and 0.11 mg/kg, respectively. The results of real-sample analyses were verified using conventional liquid-phase fluorescence spectroscopy (LPFS). Conclusion Solid-diluent type and sampling method were found to affect the performance of the SPFS technique. A combination of sodium carbonate and the powder-cell method gave the best results. According to the t-test, no difference was observed between the means obtained by SPFS and LPFS techniques in real-sample analyses.
dc.identifier.doi10.1093/jaoacint/qsad010
dc.identifier.endpage644
dc.identifier.issn1060-3271
dc.identifier.issn1944-7922
dc.identifier.issue3
dc.identifier.pmid36629445
dc.identifier.scopus2-s2.0-85159548581
dc.identifier.scopusqualityQ2
dc.identifier.startpage636
dc.identifier.urihttps://doi.org/10.1093/jaoacint/qsad010
dc.identifier.urihttps://hdl.handle.net/11480/14127
dc.identifier.volume106
dc.identifier.wosWOS:000921326900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherOxford Univ Press Inc
dc.relation.ispartofJournal of Aoac International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectFront-Face Fluorescence
dc.subjectMultimodal Hplc Separations
dc.subjectAminopropyl Silica Rplc
dc.subjectHumic-Acid
dc.subjectOrganic-Matter
dc.subjectMilk
dc.titleA Critical Study on the Potential of Solid-Phase Fluorescence Spectroscopy (SPFS) in Quantitative Analysis: Effect of Solid Diluent and Sampling Method on Signal Quality
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu