Apoptotic and cytotoxic effect of origanum minutiflorum on NB2A neuroblastoma cells

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dc.contributor.authorSari, Ismail
dc.contributor.authorDonmez Arat, Gonca
dc.contributor.authorOzkan, Oktay
dc.contributor.authorAydemir, Isil
dc.contributor.authorOzbey, Caner
dc.contributor.authorTu?lu, M. Ibrahim
dc.date.accessioned2024-11-07T10:40:13Z
dc.date.available2024-11-07T10:40:13Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractObjective: The aim of the present study is to investigate the cytotoxic and apoptotic effects of oregano oil from Origanum minutiflorum, which is an endemic medicinal plant in Turkey, on NB2a neuroblastoma cells. Material and Method: Cell proliferation, apoptosis, and expression of inducible and endothelial nitric oxide synthase have been determined by MTT, TUNEL, and immunohistochemistry, respectively. Result and Discussion: The cell viability was significantly decreased gradually in NB2a cells when the concentrations of both oregano oil and doxorubicin were increased. IC50doses were found to be10.75 ?l/ml for oregano oil and 5?M for doxorubicin. Furthermore, expression of inducible and endothelial nitric oxide synthase, also the number of apoptotic cells was significantly enhanced in NB2a cells after treatment with oregano oil and doxorubicin. There was a remarkable increase in inducible and endothelial nitric oxide synthase expression levels and in the number of apoptotic cells by the dual application of these agents. In addition, oregano oil enhanced the apoptotic effect of doxorubicin. In conclusion, our results indicated that oregano oil of O. minutiflorum has a cytotoxic and apoptotic effect on NB2a cells and these effects may be related to its enhancing effects on inducible and endothelial nitric oxide synthase levels. © 2020 University of Ankara. All rights reserved.
dc.identifier.doi10.33483/jfpau.707277
dc.identifier.endpage292
dc.identifier.issn2564-6524
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85089249574
dc.identifier.scopusqualityN/A
dc.identifier.startpage281
dc.identifier.urihttps://doi.org/10.33483/jfpau.707277
dc.identifier.urihttps://hdl.handle.net/11480/11523
dc.identifier.volume44
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherUniversity of Ankara
dc.relation.ispartofAnkara Universitesi Eczacilik Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectApoptosis
dc.subjectCytotoxicity
dc.subjectNeuroblastoma
dc.subjectNitric oxide synthase
dc.subjectOriganum minutiflorum
dc.titleApoptotic and cytotoxic effect of origanum minutiflorum on NB2A neuroblastoma cells
dc.title.alternativeORIGANUM MINUTIFLORUM'UN NB2A NÖROBLASTOMA HÜCRELERINE APOPTOTIK VE SITOTOKSIK ETKISI
dc.typeArticle

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