General guidelines for CRISPR/Cas-based genome editing in plants

Basit Öğe Kaydı
dc.authoridyildirim, kubilay/0000-0003-3834-0396
dc.authoridDemirel, Ufuk/0000-0002-3457-5086
dc.authoridSEVGEN KUCUK, ILKAY/0000-0003-1254-3515
dc.authoridyildirim, Kubilay/0000-0002-1495-5866
dc.authoridYerlikaya, Bayram Ali/0000-0002-2864-7709
dc.authoridKAVAS, MUSA/0000-0001-5903-2873
dc.contributor.authorAksoy, Emre
dc.contributor.authorYildirim, Kubilay
dc.contributor.authorKavas, Musa
dc.contributor.authorKayihan, Ceyhun
dc.contributor.authorYerlikaya, Bayram Ali
dc.contributor.authorCalik, Irmak
dc.contributor.authorSevgen, Ilkay
dc.date.accessioned2024-11-07T13:34:50Z
dc.date.available2024-11-07T13:34:50Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractCRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR-associated) technology is a versatile genome editing tool that has been used to improve agriculturally important plant traits. Due to its precision, CRISPR/Cas9 is more effective than either conventional plant breeding methods or standard genetic engineering approaches for the rapid development of new varieties resilient to climate change. In addition to knowledge in tissue culture-based plant transformation, effective gene-specific single guide RNA (sgRNA) design, prediction of its off-target effect and utilization of vectors, promoters, Cas proteins and terminators is required for CRISPR/Cas9. Various bioinformatics tools are available for the best sgRNA design and screening of the off-targets. Various tools are used in the delivery of CRISPR/Cas components into cells and the genome. Moreover, some recent studies proved the simultaneous silencing of different paralogs in the same family or several genes working in the same pathway by using multiple-target sgRNA designs. This review summarizes the type of promoters, Cas proteins, recognition sequences, and terminators available for the development of knock-out and overexpression plant lines. It also provides a general guideline for the development of genome-edited plants from the design of sgRNAs to the selection of non-transgenic genome-edited T-2 generation.
dc.identifier.doi10.1007/s11033-022-07773-8
dc.identifier.endpage12164
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue12
dc.identifier.pmid36107373
dc.identifier.scopus2-s2.0-85138089108
dc.identifier.scopusqualityQ2
dc.identifier.startpage12151
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07773-8
dc.identifier.urihttps://hdl.handle.net/11480/16187
dc.identifier.volume49
dc.identifier.wosWOS:000854738500002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectBreeding
dc.subjectCRISPR
dc.subjectGenome-editing
dc.subjectMultiple-targets
dc.subjectRNAPs
dc.subjectsgRNA
dc.titleGeneral guidelines for CRISPR/Cas-based genome editing in plants
dc.typeReview Article

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu