Sero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus

Basit Öğe Kaydı
dc.authoridAhmad, Furqan/0000-0002-5328-2174
dc.authoridMehmood, Mirza Abid/0000-0002-8171-654X
dc.authoridAshfaq, Muhammad/0000-0003-4304-3986
dc.contributor.authorSaba, Sehrish
dc.contributor.authorAshfaq, Muhammad
dc.contributor.authorMehmood, Mirza Abid
dc.contributor.authorAhmad, Furqan
dc.contributor.authorAlfarraj, Saleh
dc.contributor.authorAnsari, Mohammad Javed
dc.contributor.authorMaqbool, Amir
dc.date.accessioned2024-11-07T13:35:17Z
dc.date.available2024-11-07T13:35:17Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground: Chilli veinal mottle virus is a remarkable threat to chilli crops worldwide including Pakistan. Methods: In the present study, 18 chilli pepper genotypes were catalogued against ChiVMV ATIPK isolate by using DAS-ELISA with virus-specific polyclonal antisera. Results: Out of 18 genotypes, four genotypes viz., Loungi pepper, Sanam, Red Pepper, and CV-1 were categorized as highly susceptible followed by three susceptible (Soofi, Royal Wonder, Pepper PLSQ) and four (Yolo wonder, Bhakkar Local, CIM-506, CR-25) were moderately susceptible. The plants of these genotypes exhibited characteristic symptoms of ChiVMV and accumulated a high level of virus titer. The rest of three (California wonder, Gola Peshawar, Red top) and four (CV-7, Hot Pepper, Doni Pepper, Ghotki) genotypes were grouped as resistant and moderately resistant, respectively. Three resistant and three highly susceptible genotypes were further appraised for genetic diversity by RAPD markers and a total of 75 bands were scored, 76% of them unveiled polymorphism with an amplification range of 5-9 bands. Genetic analysis revealed 44-78.67% similarities with Mean Similarity Index (MSI) ranging from 59.46 to 65.86%. Most of the primers were highly informative with PIC value fluctuated from 0.388 to 0.666, with an average value of 0.512. Cluster analysis further confirmed and clustered both groups of genotypes in a separate clade. Conclusion: It is concluded that genotypes; California Wonder and Red Top showed diverse response from other genotypes, not only based on symptoms and ELISA but also on markers' basis. Therefore, these genotypes could be useful as ChiVMV resistant sources in the chilli breeding program. (c) 2022 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.
dc.description.sponsorshipInternational Foundation for Science (IFS), Sweden [C/5259-1]; King Saud University, Riyadh, Saudi Arabia [RSP-2023R7]
dc.description.sponsorshipThis work was funded by International Foundation for Science (IFS), Sweden under the Research Grant No. C/5259-1 to M. Ashfaq. This project was also supported by Researchers Supporting Project Number (RSP-2023R7) King Saud University, Riyadh, Saudi Arabia.
dc.identifier.doi10.1016/j.jksus.2022.102303
dc.identifier.issn1018-3647
dc.identifier.issn2213-686X
dc.identifier.issue8
dc.identifier.scopus2-s2.0-85138121744
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jksus.2022.102303
dc.identifier.urihttps://hdl.handle.net/11480/16433
dc.identifier.volume34
dc.identifier.wosWOS:000892067100014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of King Saud University Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectChilli
dc.subjectRAPD
dc.subjectGenetic diversity
dc.subjectELISA
dc.subjectChiVMV
dc.titleSero-RAPD makers based evaluation of chilli pepper germplasm for resistance to chilli veinal mottle virus
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu