Secondary structure and conformational change of mushroom polyphenol oxidase during thermosonication treatment by using FTIR spectroscopy

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dc.contributor.authorBaltacıoglu, Hande
dc.contributor.authorBayındırlı, Alev
dc.contributor.authorSevercan, Feride
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2017
dc.departmentNiğde ÖHÜ, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü
dc.description.abstractTo understand the conformational changes of mushroom PPO, the secondary structural change of the enzyme during thermosonication treatment at different power (60, 80 and 100%), temperature (20-60 degrees C) and time (0-30 min) combinations was investigated by using FTIR spectroscopy and compared with the change in enzyme activity. The enzyme inactivation higher than 99% was obtained at 100% amplitude at 60 degrees C for 10 min. FTIR studies showed that marked spectral changes were noted after ultrasound treatment at 20 degrees C. The alpha-helix and beta-sheet contents decreased, while aggregated beta p-sheet, turns and random coil contents increased as temperature increased up to 60 degrees C during thermosonication treatment for 10 min indicating protein denaturation. Aggregated bands located at 1683 and 1616 cm(-1) became evident after ultrasound treatment at 40 degrees C. When temperature was lowered back to 25 degrees C, from ultrasound treatment at 60 degrees C, these bands were still observed, indicating the irreversible change in the structure. (C) 2016 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipState Planning Organization (DPT) [BAP-08-11-DPT2002K120510-GT-4]; Scientific and Technical Research Council of the Turkish Republic (TUBITAK) [TOVAG-112O433]
dc.description.sponsorshipThis work was supported by the State Planning Organization (DPT) Grant No: BAP-08-11-DPT2002K120510-GT-4 and Scientific and Technical Research Council of the Turkish Republic (TUBITAK): Project no: TOVAG-112O433.
dc.identifier.citationSecondary structure and conformational change of mushroom polyphenol oxidase during thermosonication treatment by using FTIR spectroscopy By Baltacıoğlu, Hande; Bayındırlı, Alev; Severcan, Feride. In Food Chemistry. 1 January 2017 214:507-514 Language: English. DOI: 10.1016/j.foodchem.2016.07.021
dc.identifier.doi10.1016/j.foodchem.2016.07.021
dc.identifier.endpage514
dc.identifier.issn0308-8146
dc.identifier.issn1873-7072
dc.identifier.pmid27507504
dc.identifier.scopus2-s2.0-84978654551
dc.identifier.scopusqualityQ1
dc.identifier.startpage507
dc.identifier.urihttps://dx.doi.org/10.1016/j.foodchem.2016.07.021
dc.identifier.urihttps://hdl.handle.net/11480/3534
dc.identifier.volume214
dc.identifier.wosWOS:000384776500065
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherELSEVIER SCI LTD
dc.relation.ispartofFood Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPPO
dc.subjectThermosonication
dc.subjectFTIR
dc.subjectSpectroscopy
dc.subjectProtein Secondary Structure
dc.subjectConformation Change
dc.titleSecondary structure and conformational change of mushroom polyphenol oxidase during thermosonication treatment by using FTIR spectroscopy
dc.typeArticle

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