Molecular Characterization of Snowdrop Lectin (GNA) and its Comparison with Reported Lectin Sequences of Amaryllidaceae

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dc.authorid0000-0002-1697-9327
dc.authorid0000-0002-1697-9327
dc.authorid0000-0003-3561-7863
dc.authorid0000-0002-3091-2639
dc.contributor.authorKhabbazi, Saber Delpasand
dc.contributor.authorBakhsh, Allah
dc.contributor.authorSancak, Cengiz
dc.contributor.authorOzcan, Sebahattin
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractPlant lectins have become efficient sources of insect resistance in crops. The present study was conducted to identify, amplify, clone and characterize the plant lectin gene GNA. The lectin, present in Galanthus nivalis (snowdrop), is an agglutinin toxic to hemiptera. The attempt was made to elucidate the relationship of the lectin gene trGNA (GNA isolated and characterized from Turkey) with other previously cloned lectins having insecticidal activity and to ensure the presence of the conserved mannose-binding region/site in the gene sequence. The full-length cDNA of trGNA was 477 bp that contained a 333 bp open reading frame encoding 157 amino acid proteins with 23 amino acids of signal peptide. BLAST results showed that trGNA has 89-97% similarity with previously reported GNA sequences while it has 84-96% similarity with earlier reported GNA protein sequences. No intron was detected within the region of genomic sequence corresponding to trGNA full-length cDNA. According to the search results from the NCBI (National Centrer for Biotechnology Information database), trGNA from Galanthus nivalis is most similar to the previously reported lectin sequences of Narcissus tazetta with a similarity percentage of 87%. The obtained results are useful for engineering of plants with enhanced insecticidal activity against chewing and sucking insects, causing crop pests. In addition, medical application of lectins may also be considered.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) - BIDEB [2215]
dc.description.sponsorshipThe authors are grateful to The Scientific and Technological Research Council of Turkey (TUBITAK) - BIDEB for granting PhD. fellowship (2215) to Mr. S.D. Khabbazi.
dc.identifier.doi10.17221/58/2016-CJGPB
dc.identifier.endpage100
dc.identifier.issn1212-1975
dc.identifier.issn1805-9325
dc.identifier.issue3
dc.identifier.scopus2-s2.0-84989233207
dc.identifier.scopusqualityQ3
dc.identifier.startpage94
dc.identifier.urihttps://dx.doi.org/10.17221/58/2016-CJGPB
dc.identifier.urihttps://hdl.handle.net/11480/3761
dc.identifier.volume52
dc.identifier.wosWOS:000389669800002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherCZECH ACADEMY AGRICULTURAL SCIENCES
dc.relation.ispartofCzech Journal of Genetics and Plant Breeding
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbioinformatics
dc.subjectbiotic stress
dc.subjectGalanthus nivalis
dc.subjectgene cloning
dc.subjectgene sequence
dc.subjectinsecticidal activity
dc.titleMolecular Characterization of Snowdrop Lectin (GNA) and its Comparison with Reported Lectin Sequences of Amaryllidaceae
dc.typeArticle

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