High allelic diversity of the centromere-specific histone H3 (CENH3) in the legume sainfoin (Onobrychis viciifolia)

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dc.authoridKARA OZTURK, Sevim Dondu/0000-0001-7522-0575
dc.authoridTek, Ahmet L./0000-0002-3292-5142
dc.contributor.authorTek, Ahmet L.
dc.contributor.authorKara Ozturk, Sevim D.
dc.date.accessioned2024-11-07T13:24:23Z
dc.date.available2024-11-07T13:24:23Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe centromere is a structurally and functionally specialized region on each eukaryotic chromosome and is essential for accurate and complete segregation of chromosomes during cell division. Centromeric nucleosomes differ from canonical nucleosomes by replacement of the histone H3 with its centromere-specific variant CENH3. CENH3 is essential for active centromeres in most eukaryotes. Homologs of CENH3 are identified in many organisms. Sainfoin (Onobrychis viciifolia) is an agriculturally important perennial forage and is a legume of the Fabaceae family. There is very limited information on the structure of the sainfoin genome and no data are available on its centromere structure. Here, we aim to characterize the sainfoin CENH3 homolog (OvCENH3). Using a sequence homology-based strategy with gene-specific primers, we were able to clone transcripts from sainfoin total RNA. The amplified clones were sequenced and compared by bioinformatics tools. Four distinct alleles of OvCENH3 were detected. Our study provides the first structural features on sainfoin centromeres with a possible allotetraploid origin for sainfoin. We discuss and compare our findings with that for other important legume species.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TuBTAK) under COST Action [118Z589, CA16212]; Ayhan Sahenk Foundation; 100/2000 YoK Program
dc.description.sponsorshipWe wish to thank Prof. Ingo Schubert and anonymous reviewers for editing of the manuscript and critical suggestions. This project was funded by The Scientific and Technological Research Council of Turkey (TuBTAK) Project ID 118Z589 under the COST Action CA16212-INDEPTH (Impact of Nuclear Domains On Gene Expression and Plant Traits). SD has a fellowship from the 100/2000 YoK Program and Ayhan Sahenk Foundation.
dc.identifier.doi10.1007/s11033-020-05926-1
dc.identifier.endpage8795
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue11
dc.identifier.pmid33104994
dc.identifier.scopus2-s2.0-85093919999
dc.identifier.scopusqualityQ2
dc.identifier.startpage8789
dc.identifier.urihttps://doi.org/10.1007/s11033-020-05926-1
dc.identifier.urihttps://hdl.handle.net/11480/14072
dc.identifier.volume47
dc.identifier.wosWOS:000583940800003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectSainfoin
dc.subjectOnobrychis viciifolia
dc.subjectForage
dc.subjectLeguminosae
dc.subjectCentromere
dc.subjectCENH3
dc.titleHigh allelic diversity of the centromere-specific histone H3 (CENH3) in the legume sainfoin (Onobrychis viciifolia)
dc.typeArticle

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