L-Arginine inhibits hydrogen peroxide-induced oxidative damage and inflammatory response by regulating antioxidant capacity in ovine intestinal epithelial cells

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dc.authoridElsabagh, Mabrouk/0000-0002-0596-6547
dc.authoridZhang, Hao/0000-0002-5046-9785
dc.authoridWang, Hongrong/0000-0002-4980-1082
dc.contributor.authorZhang, Hao
dc.contributor.authorZhang, Ying
dc.contributor.authorLiu, Xiaoyun
dc.contributor.authorElsabagh, Mabrouk
dc.contributor.authorYu, Yin
dc.contributor.authorPeng, Along
dc.contributor.authorDai, Sifa
dc.date.accessioned2024-11-07T13:35:03Z
dc.date.available2024-11-07T13:35:03Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractLittle is known how L-arginine (Arg) affects the ovine intestinal epithelial cells (IOECs) redox status induced by hydrogen peroxide (H2O2)(.) This study aimed to examine the impact of Arg on IOECs subjected to H2O2-induced oxidative damage, intestinal barrier injury, and inflammatory response. The IOECs were incubated for 16 h then classified as four groups (n = 6/group) and cultured in corresponding media including (1) control (CON) group, in which IOECs were cultured in Arg-free Dulbecco's modified Eagle's F12 Ham medium (DMEM) containing 100 mu M Arg; (2) Arg group, in which IOECs were cultured in Arg-free DMEM containing 350 mu M Arg; (3) H2O2 group, in which IOECs were cultured in CON group plus 150 mu M H2O2; (4) Arg + H2O2 group, in which IOECs were cultured in Arg group plus150 mu M H2O2. After culturing for 24 h in media, some characteristics of cells in the four groups were measured. Arg administration decreased the H2O2-induced reactive oxygen species (ROS) production compared with the H2O2 group (p < .05). Compared with H2O2, adding Arg to H2O2 increased (p < .05) transepithelial electrical resistance (TEER), but decreased (p < .05) tumour necrosis factor alpha (TNF-alpha) within the IOECs. Compared with H2O2, adding Arg to H(2)O(2-)induced injured IOECs increased (p < .05) glutathione peroxidase 1 (GPx1), zonula occludens-1 (ZO-1), and epithelial nitric oxide (NO) synthase (eNOS) protein levels, and decreased (p < .05) TNF-alpha levels within cells. Arg inhibits H2O2-induced oxidative damage, intestinal barrier injury, and inflammatory response by NO pathway within IOECs.
dc.description.sponsorshipNational Natural Science Foundation of China [31902180, 32160799]; Key R&D project of Jiangxi Province [20203BBF63021]; Top Talents Award Plan of Yangzhou University; Cyanine Project of Yangzhou University
dc.description.sponsorshipThe research was supported by the fund for the National Natural Science Foundation of China [31902180], the National Natural Science Foundation of China [32160799], the Key R&D project of Jiangxi Province [20203BBF63021], the Top Talents Award Plan of Yangzhou University (2019), and the Cyanine Project of Yangzhou University (2020).
dc.identifier.doi10.1080/1828051X.2021.1973916
dc.identifier.endpage1632
dc.identifier.issn1594-4077
dc.identifier.issn1828-051X
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85117420113
dc.identifier.scopusqualityQ1
dc.identifier.startpage1620
dc.identifier.urihttps://doi.org/10.1080/1828051X.2021.1973916
dc.identifier.urihttps://hdl.handle.net/11480/16309
dc.identifier.volume20
dc.identifier.wosWOS:000709139500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofItalian Journal of Animal Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectOvine intestinal epithelial cells
dc.subjectL-arginine
dc.subjectOxidative damage
dc.subjectIntestinal barrier injury
dc.subjectInflammatory response
dc.subjectNO pathway
dc.titleL-Arginine inhibits hydrogen peroxide-induced oxidative damage and inflammatory response by regulating antioxidant capacity in ovine intestinal epithelial cells
dc.typeArticle

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