Morphological and Biochemical Changes in the Mediterranean Cereal Cyst Nematode (Heterodera latipons) during Diapause

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dc.authoridSalem, Nida'/0000-0003-3778-2213
dc.authoridDABABAT, Abdelfattah/0000-0002-3172-0452
dc.authoridSadder, Monther/0000-0001-6651-0423
dc.contributor.authorAbumuslem, Motasem
dc.contributor.authorToktay, Halil
dc.contributor.authorSadder, Monther T.
dc.contributor.authorDababat, Abdelfattah A.
dc.contributor.authorSalem, Nida' M.
dc.contributor.authorAL-Banna, Luma
dc.date.accessioned2024-11-07T13:34:33Z
dc.date.available2024-11-07T13:34:33Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe cereal cyst nematode (Heterodera latipons) is becoming an economically important species in global cereal production as it is being identified in many new cereal cultivated areas and causes significant losses. Consequently, understanding its biology becomes crucial for researchers in identifying its vulnerabilities and implementing effective control measures. In the current study, different morphological and biochemical changes of H. latipons cysts containing eggs with infective juveniles from a barley field in Jordan were studied during the summer of 2021, at two sample dates. The first, at the harvest of the cereal crop (June 2021), when the infective second-stage juveniles (J2s) were initiating diapause, and the second, before planting the sequent cereal crop (late October 2021), when the J2s were ending diapause. The studied population was characterized morphologically and molecularly, showing 98.4% molecular similarity to both JOD from Jordan and Syrian 300 isolates of H. latipons. The obtained results and observations revealed that there were dramatic changes in all the investigated features of the cysts and eggs they contained. Morphological changes such as cyst color, sub-crystalline layer, and thickness of the rigid eggshell wall were observed. A slight change in the emergence time of J2s from cysts was observed without any difference in the number of emerged J2s. The results of biochemical changes showed that the total contents of carbohydrates, glycogen, trehalose, glycerol, and protein were higher in cysts collected in October when compared to those cysts collected in June. The SDS-PAGE pattern indicated the presence of a protein with the size of ca. 100 kDa in both sampling dates, whereas another protein (ca. 20 kDa) was present only in the cysts of October. Furthermore, the expression of trehalase (tre) gene was detected only in H. latipons collected in October. The outcomes of this study provide new helpful information that elucidates diapause in H. latipons and may be used for the implementation of new management strategies of cyst nematodes.
dc.description.sponsorshipInternational Maize and Wheat Improvement Center (CIMMYT)
dc.description.sponsorshipThis research was partially funded by the International Maize and Wheat Improvement Center (CIMMYT).
dc.identifier.doi10.3390/pathogens13080656
dc.identifier.issn2076-0817
dc.identifier.issue8
dc.identifier.pmid39204256
dc.identifier.scopus2-s2.0-85202460625
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.3390/pathogens13080656
dc.identifier.urihttps://hdl.handle.net/11480/16048
dc.identifier.volume13
dc.identifier.wosWOS:001307096600001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMdpi
dc.relation.ispartofPathogens
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectenzyme
dc.subjectgenes
dc.subjectmetabolism
dc.subjectmolecular characterization
dc.subjectmorphometrics
dc.subjecttrehalase
dc.titleMorphological and Biochemical Changes in the Mediterranean Cereal Cyst Nematode (Heterodera latipons) during Diapause
dc.typeArticle

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