Exploring non-cytotoxic, antioxidant, and anti-inflammatory properties of selenium nanoparticles synthesized from Gymnema sylvestre and Cinnamon cassia extracts for herbal nanomedicine

Basit Öğe Kaydı
dc.authoridAli, Dr. Habib/0000-0003-4650-0345
dc.contributor.authorBi, Sumairan Bi
dc.contributor.authorElahi, Iqra
dc.contributor.authorSardar, Nimra
dc.contributor.authorGhaffar, Omer
dc.contributor.authorAli, Habib
dc.contributor.authorAlsubki, Roua A.
dc.contributor.authorIqbal, Muhammad Sarfaraz
dc.date.accessioned2024-11-07T13:35:25Z
dc.date.available2024-11-07T13:35:25Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe increasing need for pharmaceutical agents that possess attributes such as safety, cost-effectiveness, environmental sustainability, and absence of side effects has driven the advancement of nanomedicine research, which lies at the convergence of nanotechnology and medicine. Aims and objectives: The study aimed to synthesize non-toxic selenium nanoparticles (SeNPs) using Gymnema sylvestre (G. sylvestre) and Cinnamon cassia (C. cassia) extracts. It also sought to develop and evaluate versatile nanomedicine formulations i.e. selenium nanoparticles of G. sylvestre and C. cassia (SeNPs), drug (lupeol) loaded SeNPs (DLSeNPs), drug-loaded and coated (PEG) SeNPs (DLCSeNPs) without side effects. Methods: The SeNPs formulations were hydrothermally synthesized, loaded with lupeol to improve efficacy, coated with polyethylene glycol (PEG) for targeted delivery, and characterized using UV-Vis spectrophotometry, Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), zeta potential analysis, size distribution analysis, and X-ray diffraction (XRD). Hemolytic cytotoxicity, 2,2-Diphenyl-1-picrylhydzayl (DPPH), total Reducing power, and total antioxidant capacity (TAC) antioxidant assays, carrageenan-induced paw edema, and histological studies were used to estimate the acute anti-inflammatory activity of the synthesized SeNPs. Results: The final form of PEGylated and drug (lupeol)-loaded selenium nanoparticles (DLCSeNPs) exhibited an average particle size ranging from 100 to 500 nm as evidenced by SEM, and Zeta potential results. These nanoparticles demonstrated no cytotoxic effects and displayed remarkable antioxidant (IC50 values 19.29) and anti-inflammatory capabilities. These results were fed into Graph-pad Prism 5 software and analyzed by one-way ANOVA, followed by Tukey's post hoc test (p < 0.001). All nano-formulations exhibited significant overall antioxidant activity, with IC50 values <= 386 (p < 0.05) as analyzed by ANOVA. The study's results suggest that G. sylvestre outperformed C. cassia in terms of reducing 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical, potassium ferricyanide, and ammonium molybdate in respective antioxidant assays. As far as antiinflammatory activities are concerned drug (lupeol)-loaded and PEG-coated G. sylvestre SeNPs exhibited the highest anti-inflammatory potential from all other nano-formulations including drug (lupeol)-loaded and PEGcoated C. cassia SeNPs, as exhibited to reduce the release of pro-inflammatory signals i.e. cytokines and NFkB, making them innovative anti-inflammatory nanomedicine. Conclusion: The study synthesized lupeol-loaded and PEG-coated SeNPs, showcasing the potential for biocompatible, cost-effective anti-inflammatory nanomedicines. G. Sylvester's superior antioxidant and antiinflammatory performance than Cinnamon cassia emphasizes medicinal plant versatility.
dc.description.sponsorshipKing Saud University, Riyadh, Saudi Arabia [RSP-2024 R369]
dc.description.sponsorshipThe authors extend their appreciation to Researchers Supporting Project number (RSP-2024 R369) , King Saud University, Riyadh, Saudi Arabia. I want to express my sincere gratitude to Dr. Farkhanda Yasmeen for taking the lead in initiating the writing of this article.
dc.identifier.doi10.1016/j.micpath.2024.106670
dc.identifier.issn0882-4010
dc.identifier.issn1096-1208
dc.identifier.pmid38734323
dc.identifier.scopus2-s2.0-85193442071
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.micpath.2024.106670
dc.identifier.urihttps://hdl.handle.net/11480/16497
dc.identifier.volume192
dc.identifier.wosWOS:001244495900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.relation.ispartofMicrobial Pathogenesis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectSelenium nanoparticles
dc.subjectGreen synthesis
dc.subjectPEGylated nano-carriers
dc.subjectTargeted drug delivery
dc.subjectGymnema sylvestre
dc.subjectCinnamon cassia
dc.subjectAntioxidant potential
dc.subjectAnti-Inflammatory agents
dc.titleExploring non-cytotoxic, antioxidant, and anti-inflammatory properties of selenium nanoparticles synthesized from Gymnema sylvestre and Cinnamon cassia extracts for herbal nanomedicine
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu