L-Arginine Alleviates Hydrogen Peroxide-Induced Oxidative Damage in Ovine Intestinal Epithelial Cells by Regulating Apoptosis, Mitochondrial Function, and Autophagy

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dc.authoridElsabagh, Mabrouk/0000-0002-0596-6547
dc.authoridWang, Hongrong/0000-0002-4980-1082
dc.contributor.authorZhang, Hao
dc.contributor.authorLiu, Xiaoyun
dc.contributor.authorFan, Yaotian
dc.contributor.authorYu, Yin
dc.contributor.authorLoor, Juan J.
dc.contributor.authorElsabagh, Mabrouk
dc.contributor.authorPeng, Along
dc.date.accessioned2024-11-07T13:35:13Z
dc.date.available2024-11-07T13:35:13Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground: Previous studies demonstrated that dietary L-arginine (Arg) alters the equilibrium between reactive oxygen species (ROS) generation and biological defenses to resist oxidant-induced toxicity. Whether supplying Arg can protect ovine intestinal epithelial cells (OIECs) from hydrogen peroxide (H2O2)-induced oxidative damage is unclear. Objectives: The current study aimed to examine the effect of Arg on mitophagy, mitochondrial dysfunction, and apoptosis induced by H2O2 in OIECs. Methods: The OIECs were incubated in Arg-free DMEM supplemented with 100 AM Arg (CON) or 350 AM Arg (ARG) alone or with 150 mu M H2O2 (CON + H2O2, ARG + H2O2) for 24 h. Cellular apoptosis, mitochondrial function, autophagy, and the related categories of genes and proteins were determined. All data were analyzed by ANOVA using the general linear model procedures of SAS (SAS Institute) for a 2 x 2 factorial design. Results: Relative to the CON and ARG groups, H2O2 administration resulted in 44.9% and 26.5% lower (P < 0.05) cell viability but 34.7% and 61.8% greater (P < 0.05) ROS concentration in OIECs, respectively. Compared with the CON and CON + H2O2 groups, Arg supplementation led to 40.7% and 28.8% lower (P < 0.05) ROS concentration but 14.9%-49.0% and 29.3%-64.1% greater (P < 0.05) mitochondrial membrane potential, relative mitochondrial DNA content, and complex (I-IV) activity in OIECs, respectively. Compared with the CON and CON + H2O2 groups, Arg supplementation led to 33.9%-53.1% and 22.4%-49.1 % lower (P < 0.05) mRNA abundance of proapoptotic genes, respectively. Relative to the CON and CON + H2O2 groups, Arg supplementation resulted in 33.0%-59.2% and 14.6%-37.7% lower (P< 0.05) abundance of proapoptotic, mitophagy, and cytoplasmic cytochrome c protein, respectively. Conclusions: Supply of Arg protects OIECs against H2O2-induced damage partly by improving mitochondrial function and alleviating cellular apoptosis and autophagy.
dc.description.sponsorshipNational Natural Science Foundation of China [31902180]; Research Project of Natural Science Foundation of Jiangsu Province [BK20170488]; China Postdoctoral Science Foundation [2017M610358]; Top Talents Award Plan of Yangzhou University; Cyanine Project of Yangzhou University; Science and Technology Innovation Project of Yangzhou University [2019CXJ152]
dc.description.sponsorshipSupported by National Natural Science Foundation of China grant 31902180, Research Project of Natural Science Foundation of Jiangsu Province grant BK20170488, China Postdoctoral Science Foundation grant 2017M610358, Top Talents Award Plan of Yangzhou University (2019), Cyanine Project of Yangzhou University (2020), and Science and Technology Innovation Project of Yangzhou University grant 2019CXJ152.
dc.identifier.doi10.1093/jn/nxaa428
dc.identifier.endpage1046
dc.identifier.issn0022-3166
dc.identifier.issn1541-6100
dc.identifier.issue4
dc.identifier.pmid33693729
dc.identifier.scopus2-s2.0-85104160490
dc.identifier.scopusqualityQ1
dc.identifier.startpage1038
dc.identifier.urihttps://doi.org/10.1093/jn/nxaa428
dc.identifier.urihttps://hdl.handle.net/11480/16391
dc.identifier.volume151
dc.identifier.wosWOS:000638012000014
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherOxford Univ Press
dc.relation.ispartofJournal of Nutrition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectL-arginine
dc.subjectoxidative stress
dc.subjectintestinal epithelial cells
dc.subjectmitochondrial function
dc.subjectmitophagy
dc.titleL-Arginine Alleviates Hydrogen Peroxide-Induced Oxidative Damage in Ovine Intestinal Epithelial Cells by Regulating Apoptosis, Mitochondrial Function, and Autophagy
dc.typeArticle

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