Tissue-specific and stress-inducible promoters establish their suitability for containment of foreign gene(s) expression in transgenic potatoes

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dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.contributor.authorRahamkulov, Ilhom
dc.contributor.authorBakhsh, Allah
dc.date.accessioned2024-11-07T13:24:44Z
dc.date.available2024-11-07T13:24:44Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe present study was conducted to determine efficiency of green tissue-specific (pRCA) and stress-inducible promoters (pRD29A) to expressE. colibeta-glucuronidase (gusA) gene in transgenic potatoes compared with constitutive promoter (35S CaMV). The promoter fragments were isolated from their original source and cloned upstream togusAin pCAMBIA-1301 binary vector to develop plant expression constructs, i.e., pRCA-pCAMBIA and pRD29A-pCAMBIA.Agrobacteriumstrain GV2260 harboring recombinant plasmids were used to infect leaf discs and internodal explant of Lady Olympia cultivar. GUS histochemical analysis was performed at different stages to determine GUS activity in transgenic plants. To determine activity of stress-inducible promoter (pRD29A), transgenic plants were exposed to heat, drought and combination of both heat and drought stress. The real time (RT-qPCR) and GUS florimetric assays revealed that pRD29A promoter gets more activated under drought, heat and combination of both stresses. GUS expression levels were more than 10 folds high with pRD29A promoter compared to control. Likewise, the reduced transcripts levels ofgusAgene under control of pRCA promoter were found in tuber/roots of transgenic plants compared to 35S promoter. GUS florimetric assays also showed decreased or no GUS expression in tubers. In conclusion, the results encourage the appropriate use of promoters to drive the expression of foreign gene(s) for the development of potato lines tolerant to biotic and abiotic stress while minimizing the risks of transgenic technology in potatoes.
dc.description.sponsorshipTUBITAK [215O520]; Tubitak
dc.description.sponsorshipThe present study is a part of research project supported by TUBITAK (Grant No. 215O520), where we used pRCA promoter to drive the expression of EcR dsRNAs in green part of potato plants. The authors are highly thankful to Tubitak for providing fellowship to Mr. Ilhom Rahamkulov from the project budget. Authors are grateful to Prof. Dr. Mehmet Emin Caliskan for providing seed tuber of Lady Olympia used in the study. Special thanks to Dr. Emre Aksoy for his technical help during experiments and interpretation of results. We are also thankful to Dr. Halil Toktay for providing access to microscope facility.
dc.identifier.doi10.1007/s13205-020-02350-x
dc.identifier.issn2190-572X
dc.identifier.issn2190-5738
dc.identifier.issue10
dc.identifier.pmid32968611
dc.identifier.scopus2-s2.0-85090559771
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s13205-020-02350-x
dc.identifier.urihttps://hdl.handle.net/11480/14260
dc.identifier.volume10
dc.identifier.wosWOS:000571783400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartof3 Biotech
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectPromoter efficiency
dc.subjectPotato
dc.subjectTransgene development
dc.subjectTargeted expression
dc.titleTissue-specific and stress-inducible promoters establish their suitability for containment of foreign gene(s) expression in transgenic potatoes
dc.typeArticle

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