Pretreatment with spermidine and proline reverses inhibitory effects of Phytophthora capsici stress in pepper

Basit Öğe Kaydı
dc.authorid0000-0001-6820-0381
dc.contributor.authorKoc, Esra
dc.contributor.authorArici, Yeliz Kasko
dc.contributor.authorIslek, Cemil
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractPhytophthora blight (Phytophthora capsici Leon.), which is one of the most important disease factors incurring great economic losses, causes root crown rot. When pepper seedlings were pretreated with 0.1 mM spermidine (spd), 1 mM spd, 0.1 mM spd + 1 mM proline, 0.1 mM spd + 10 mM proline, 1 mM spd + 1 mM proline and 1 mM spd + 10 mM proline and then exposed to P. capsici, they improved their tolerance to subsequent P. capsici-induced oxidative damage. It was detected that cultivar 'Sirena RZ F1' was resistant to P. capsici and it was determined that cultivar 'Sera Demre-8' exhibited sensitive reaction. P. capsici stress caused an increase in total phenolic, malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels, as well as peroxidase (POX) and polyphenol oxidase (PPO) activities in leaves of pepper seedlings. Application of exogenous spermidine and spermidine + proline further increased PPO and POX activity, total phenolic amount. On the other hand, these applications in two cultivars and all days decreased the disease severity, necrosis length and MDA and H2O2 amounts. These data show that antioxidants, especially polyphenol oxidase, peroxidase and total phenolic appear to protect pepper seedlings against stress-related damage. Moreover, spermidine and spermidine + proline have the potential to scavenge directly free radicals and promote the activity of antioxidant enzymes in pepper seedlings under P. capsici stress. In addition, it was also identified that especially 0.1 mM spd, 1 mM spd and 0.1 mM spd + 1 mM proline applications decreased the colony growth of P. capsici and the amount of zoospores. Therefore, we suggest that spermidine and spermidine + proline could play a protective role in protecting the pepper seedlings from P. capsici stress.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112T725]
dc.description.sponsorshipThis study is a part of the project (No. 112T725) financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK).
dc.identifier.doi10.13080/z-a.2016.103.053
dc.identifier.endpage418
dc.identifier.issn1392-3196
dc.identifier.issue4
dc.identifier.scopus2-s2.0-84995968762
dc.identifier.scopusqualityQ3
dc.identifier.startpage411
dc.identifier.urihttps://dx.doi.org/10.13080/z-a.2016.103.053
dc.identifier.urihttps://hdl.handle.net/11480/3758
dc.identifier.volume103
dc.identifier.wosWOS:000390417800011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherLITHUANIAN RESEARCH CENTRE AGRICULTURE & FORESTRY
dc.relation.ispartofZEMDIRBYSTE-AGRICULTURE
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectantioxidants
dc.subjectCapsicum annuum
dc.subjectdisease severity
dc.subjectnecrosis
dc.subjecttolerance
dc.titlePretreatment with spermidine and proline reverses inhibitory effects of Phytophthora capsici stress in pepper
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu