Dietary supplementation of l-arginine and N-carbamylglutamate enhances duodenal barrier and mitochondrial functions and suppresses duodenal inflammation and mitophagy in suckling lambs suffering from intrauterine-growth-restriction

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dc.authoridWang, Hongrong/0000-0002-4980-1082
dc.authoridLoor, Juan/0000-0003-1586-4365
dc.authoridZhang, Hao/0000-0002-5046-9785
dc.authoridElsabagh, Mabrouk/0000-0002-0596-6547
dc.contributor.authorZhang, Hao
dc.contributor.authorMa, Yi
dc.contributor.authorWang, Mengzhi
dc.contributor.authorElsabagh, Mabrouk
dc.contributor.authorLoor, Juan J.
dc.contributor.authorWang, Hongrong
dc.date.accessioned2024-11-07T13:35:12Z
dc.date.available2024-11-07T13:35:12Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe current work aimed at investigating the effects of the dietary supplementation of N-carbamylglutamate (NCG) or l-arginine (Arg) on the duodenal mitophagy, mitochondrial function, inflammation, and barrier function in suckling lambs suffering from intrauterine-growth-retardation (IUGR). Forty-eight neonate Hu lambs were used in this study: 12 lambs with normal birth weight (NBW: 4.25 +/- 0.14 kg) and 36 lambs with IUGR (3.01 +/- 0.13 kg). Seven day old lambs were assigned to 4 treatment groups (12 lambs in each group) as follows: control group (CON), IUGR group, IUGR + Arg, and IUGR + NCG. Lambs were fed the experimental diets for 21 days from 7 days to 28 days of age. Compared with IUGR lambs, the Arg or NCG-treated IUGR lambs had a markedly higher duodenal transepithelial electrical resistance (TER) and lower fluorescein isothiocyanate dextran (FD4) (P < 0.05), respectively. The duodenal mitochondrial membrane potential change (Delta psi(m)), relative mitochondrial DNA (mtDNA) content, adenosine triphosphate (ATP) level, together with the activities of the respiratory complexes I, III, and IV were markedly higher in Arg or NCG-treated IUGR lambs than those in non-supplemented IUGR lambs (P < 0.05). The expressions of the integrity-related proteins (occludin and zonula occludens-1 (ZO-1)), antioxidant- and apoptosis-related proteins (B-cell lymphoma/leukaemia 2 (Bcl2), superoxide dismutase 2 (SOD2), catalase (CAT), and glutathione peroxidase 1 (GPx1)), and the nitric oxide-dependent pathway-related proteins (epithelial NO synthase (eNOS) and inducible NO synthase (iNOS)) were higher in NCG or Arg-supplemented IUGR lambs than those in nontreated IUGR lambs (P < 0.05). The duodenal expressions of the mitophagy-related proteins (microtubule-associated protein light chain 3 (LC3) I, LC3 II, Belin1, PTEN induced putative kinase 1 (PINK1), and Parkin) and the immune function-related proteins (myeloid differentiation factor 88 (MyD88), IL-6, nuclear factor kappa B (p65), toll-like receptor (TLR4) and TNF-alpha) were reduced (P < 0.05) in NCG or Arg-supplemented IUGR lambs compared with non-supplemented IUGR lambs. These results demonstrated that the dietary supplementation of Arg or NCG enhanced the duodenal barrier function and mitochondrial function, mitigated duodenal inflammation, and suppressed mitophagy in suckling lambs suffering from IUGR.
dc.description.sponsorshipfund for the National Natural Science Foundation of China [31902180]; Research Project of the Natural Science Foundation of Jiangsu Province [BK20170488]; China Postdoctoral Science Foundation [2017M610358]
dc.description.sponsorshipThe research was supported by the fund for the National Natural Science Foundation of China (grant number 31902180), the Research Project of the Natural Science Foundation of Jiangsu Province (BK20170488), and the China Postdoctoral Science Foundation (2017M610358). The authors thank all the members of the Hong Rong Wang's laboratory for their contribution to sample determination.
dc.identifier.doi10.1039/d0fo00019a
dc.identifier.endpage4470
dc.identifier.issn2042-6496
dc.identifier.issn2042-650X
dc.identifier.issue5
dc.identifier.pmid32374309
dc.identifier.scopus2-s2.0-85085535163
dc.identifier.scopusqualityQ1
dc.identifier.startpage4456
dc.identifier.urihttps://doi.org/10.1039/d0fo00019a
dc.identifier.urihttps://hdl.handle.net/11480/16361
dc.identifier.volume11
dc.identifier.wosWOS:000538041500055
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofFood & Function
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectReactive Oxygen
dc.subjectNitric-Oxide
dc.subjectIntestinal Permeability
dc.subjectOxidative Stress
dc.subjectEpithelial-Cells
dc.subjectAmino-Acids
dc.subjectExpression
dc.subjectReceptor
dc.subjectApoptosis
dc.subjectAutophagy
dc.titleDietary supplementation of l-arginine and N-carbamylglutamate enhances duodenal barrier and mitochondrial functions and suppresses duodenal inflammation and mitophagy in suckling lambs suffering from intrauterine-growth-restriction
dc.typeArticle

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