Immobilization of laccase onto a porous nanocomposite: application for textile dye degradation

Küçük Resim Yok

Tarih

2016

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

SCIENTIFIC TECHNICAL RESEARCH COUNCIL TURKEY-TUBITAK

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Poly(MA-alt-MVE)-g-PLA/ODA-MMT nanocomposite was prepared by self-catalytic interlamellar graft copolymerization of L-lactic acid (LA) onto poly(maleic anhydride-alt-methyl vinyl ether) copolymer in the presence of octadecyl amine-montmorillonite (ODA-MMT) organoclay. FTIR, H-1 (C-13) NMR, XRD, and SEM-TEM were utilized for characterizing the resultant nanocomposite. Lactase from Trametes versicolor was immobilized onto the prepared nanocomposite by adsorption or covalent coupling. Decolorization of Reactive Red 3 from aqueous solution by laccase immobilized on the nanocomposite was studied in different conditions (pH, temperature, dye concentration, and reaction time) to investigate the decolorization activity with repeated use and storage. The results indicated that more than 77% of the activity of laccase immobilized systems was retained at the end of 10 cycles. The final decolorization capacity of the immobilized laccase was significantly higher (65%) than that of free laccase (33%) in the chosen optimized conditions (pH 5, 20 degrees C, 0.05 mg/mL laccase concentration, and 90 min).

Açıklama

Anahtar Kelimeler

Nanocomposite, functional copolymer, interlamellar graft copolymerization, enzyme laccase, immobilization, reactive dyes, decolorization

Kaynak

TURKISH JOURNAL OF CHEMISTRY

WoS Q Değeri

Q3

Scopus Q Değeri

Q3

Cilt

40

Sayı

2

Künye