Encapsulation of SE-P47 phage specific to Salmonella Enteritidis and evaluation of its stability

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dc.contributor.authorAlasalvar, Gamze Kocer
dc.contributor.authorYildirim, Zeliha
dc.date.accessioned2024-11-07T13:24:13Z
dc.date.available2024-11-07T13:24:13Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThis study was conducted to encapsulate the bacteriophage SE-P47 target to Salmonella Enteritidis by extrusion method and to examine its stability. Screening and optimization of effective factors in the encapsulation process were performed using fractional factorial design and face-centered central composite design, respectively. The optimum Na-alginate concentration and the ratio of coating material to phage for the encapsulation of SE-P47 phage were 1.5% (v/v) and 2:1, respectively. At the optimum encapsulation point, the highest encapsulation efficiency (98.52%), smallest capsule size (1.28 mm), and highest phage titer were achieved. When exposed to heat treatment at 80 degrees C for 30 min, pH 2 and simulated gastric fluid (pH 2.0) for 120 min, the encapsulated phage maintained almost its stability, but the free (non-encapsulated) phage almost lost its activity. Phage release from beads in simulated intestinal fluid reached 100% in 4 h. In addition, free and encapsulated phage completely maintained their activity for 12 months at 4 and 25 degrees C. The Salmonella phage encapsulated in this study exhibits high stability in harsh conditions. Thus, this encapsulated phage has the potential to be used as a biocontrol agent or therapeutic purposes in the food chain.
dc.description.sponsorshipNIGDE OMER HALISDEMIR UNIVERSITY
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1111/jfs.13103
dc.identifier.issn0149-6085
dc.identifier.issn1745-4565
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85183706569
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1111/jfs.13103
dc.identifier.urihttps://hdl.handle.net/11480/13969
dc.identifier.volume44
dc.identifier.wosWOS:001155960100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Food Safety
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectbacteriophage
dc.subjectencapsulation
dc.subjectoptimization
dc.subjectSalmonella
dc.subjectstability
dc.titleEncapsulation of SE-P47 phage specific to Salmonella Enteritidis and evaluation of its stability
dc.typeArticle

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