Coenzyme Q10 and Vitamin E Regulate the Bioactivity of Human Corneal Fibroblast Cells

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dc.authoridBozkurt, Serife Buket/0000-0002-8641-2844
dc.contributor.authorZor, Kursad Ramazan
dc.contributor.authorYilmaz, Ugur
dc.contributor.authorBozkurt, Serife Buket
dc.date.accessioned2024-11-07T13:32:23Z
dc.date.available2024-11-07T13:32:23Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPurpose: Corneal fibroblasts are involved in the wound healing of the cornea with proliferation, migration, and differentiation processes. Coenzyme Q10 (CoQ10) and vitamin E can enhance corneal wound healing when applied after a corneal lesion as an eye drop. Thus, this study was performed to determine the potential efficiency of a CoQ10 ophthalmical solution containing a CoQ10 and vitamin E D-alpha-tocopherol polyethylene glycol 1000 succinate (TPGS)-derived formulation in human corneal fibroblasts (HCFs) in vitro.Methods: Primary HCFs were obtained from cadaveric corneal tissue, and cell viability was determined using MTT assay at 24 and 72 h. Cell migration was evaluated using an in vitro wound healing assay, and mRNA expressions of collagen type I (COL-I), collagen type III (COL-III), lumican, hyaluronan, matrix metalloproteinase (MMP)-1, MMP-2, MMP-9, tissue inhibitors of MMP (TIMP)-1, TIMP-2, interleukin (IL)-1 beta, IL-6, IL-8, and IL-10 were assessed using reverse transcription polymerase chain reaction at 24 and 72 h.Results: At various concentrations of CoQ10 ophthalmical solution (CoQ10-os), cell viability and wound healing rates of HCFs increased compared with the control group. The expressions of COL-I, COL-III, lumican, and hyaluronan were increased by CoQ10-os, whereas those of MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2, and TIMP-3 were not affected by CoQ10-os at 24 and 72 h. In treating HCFs with a CoQ10-os medium, IL-1 beta, IL-6, and IL-8 decreased, whereas IL-10 was significantly increased in a time- and dose-dependent manner.Conclusions: The findings indicate that CoQ10 and vitamin E-TPGS are potent regulators of the bioactivity of HCFs, thus supporting their potential application as ophthalmical solutions in therapies aimed at the fast regeneration of damaged cornea tissues.
dc.identifier.doi10.1089/jop.2024.0050
dc.identifier.endpage503
dc.identifier.issn1080-7683
dc.identifier.issn1557-7732
dc.identifier.issue8
dc.identifier.pmid38976309
dc.identifier.scopus2-s2.0-85198648311
dc.identifier.scopusqualityQ2
dc.identifier.startpage494
dc.identifier.urihttps://doi.org/10.1089/jop.2024.0050
dc.identifier.urihttps://hdl.handle.net/11480/15363
dc.identifier.volume40
dc.identifier.wosWOS:001264188600001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofJournal of Ocular Pharmacology and Therapeutics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectcell viability
dc.subjectCoQ10
dc.subjecthuman corneal fibroblast
dc.subjectmRNA expression
dc.subjectvitamin E
dc.titleCoenzyme Q10 and Vitamin E Regulate the Bioactivity of Human Corneal Fibroblast Cells
dc.typeArticle

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