Subcellular localization and developmental regulation of cytosolic, soluble pyrophosphatase isoforms in Arabidopsis thaliana

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dc.contributor.authorOzturk, Zahide Neslihan
dc.contributor.authorGreiner, Steffen
dc.contributor.authorRausch, Thomas
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2014
dc.departmentNiğde ÖHÜ
dc.description.abstractPyrophosphate (PPi) is the by-product of several reversible key reactions of primary metabolism. Thus, generated PPi should be removed by hydrolysis via pyrophosphatases to prevent accumulation and to drive anabolism. In plastids, a plastidic, soluble pyrophosphatase splits PPi released by ADPG pyrophosphorylase. The cytosolic PPi pool is believed to be hydrolyzed by tonoplast- and/or Golgi-integral H+-translocating pyrophosphatases. The Arabidopsis thaliana (L.) Heynh. genome encodes 6 soluble pyrophosphatase isoforms (PPas), 1 of which was shown to be localized in plastids. The remaining 5 of those PPas (PPas 1-5) are more similar to each other with highly conserved protein sequences. They all lack known targeting sequences and are thought to reside in the cytosol. To address their role and redundancy in plants, we performed (i) subcellular targeting of C-terminal PPa:EGFP fusions, (ii) analysis of transgenic promoter beta-glucuronidase (GUS) lines to depict differential expression during plant development, and (iii) transcript quantification via real-time PCR to corroborate promoter-GUS data. The results revealed pronounced tissue specificity and developmental regulation for each PPa isoform, but also partial redundancy with coexpression of several PPa isoforms in all plant tissues.
dc.identifier.doi10.3906/bot-1403-67
dc.identifier.endpage1049
dc.identifier.issn1300-008X
dc.identifier.issn1303-6106
dc.identifier.issue6
dc.identifier.scopus2-s2.0-84934272350
dc.identifier.scopusqualityQ2
dc.identifier.startpage1036
dc.identifier.urihttps://dx.doi.org/10.3906/bot-1403-67
dc.identifier.urihttps://hdl.handle.net/11480/4227
dc.identifier.volume38
dc.identifier.wosWOS:000345129500002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherTUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY
dc.relation.ispartofTURKISH JOURNAL OF BOTANY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBrassicaceae
dc.subjectCruciferae
dc.subjectsoluble pyrophosphatase family
dc.subjectin silico subcellular localization
dc.subjectEGFP chimeras
dc.subjecthistochemical GUS staining
dc.subjectreal-time PCR
dc.titleSubcellular localization and developmental regulation of cytosolic, soluble pyrophosphatase isoforms in Arabidopsis thaliana
dc.typeArticle

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