Downregulation of imidacloprid resistant genes alters the biological parameters in Colorado potato beetle, Leptinotarsa decemlineata Say (chrysomelidae: Coleoptera)

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dc.authoridGokce, Ayhan/0000-0002-5617-1349
dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridNaqqash, Muhammad Nadir/0000-0001-8774-7358
dc.contributor.authorNaqqash, Muhammad Nadir
dc.contributor.authorGokce, Ayhan
dc.contributor.authorAksoy, Emre
dc.contributor.authorBakhsh, Allah
dc.date.accessioned2024-11-07T13:25:14Z
dc.date.available2024-11-07T13:25:14Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractColorado potato beetle, Leptinotarsa decemlineata Say (coleoptera: chrysomelidae), is the important pest of potato all over the world. This insect pest is resistant to more than 50 active compounds belonging to various chemical groups. Potential of RNA interference (RNAi) was explored to knock down transcript levels of imidacloprid resistant genes in Colorado potato beetle (CPB) under laboratory conditions. Three important genes belonging to cuticular protein (CP), cytochrome P450 monoxygenases (P450) and glutathione synthetase (GSS) families encoding imidacloprid resistance were targeted. Feeding bioassays were conducted on various stages of imidacloprid resistant CPB lab population by applying HT115 expressing dsRNA on potato leaflets. Survival rate of insects exposed to CP-dsRNA decreased to 4.23%, 1532% and 47.35% in 2nd, 3rd and 4th instar larvae respectively. Larval weight and pre-adult duration were also affected due to dsRNAs feeding. Synergism of RNAi with imidacloprid conducted on the 2nd instar larvae, exhibited 100% mortality of larvae when subjected to reduced doses of GSS and CP dsRNAs along with imidacloprid. Utilization of three different dsRNAs against imidacloprid resistant CPB population reveal that dsRNAs targeting CP, P450 and GSS enzymes could be useful tool in management of imidacloprid resistant CPB populations. (C) 2019 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipDogu Ar-Ge; Ttibitak
dc.description.sponsorshipWe acknowledge Dogu Ar-Ge for providing funds for the study and Ttibitak for providing PhD fellowship to Mr. Muhammad Nadir Naqqash. Authors are grateful to Dr. Muhammad Salim for his help in conducting experiments and data analysis. We also acknowledge Prof. Dr. Hsin Chi for his support and technical guidance during research work.
dc.identifier.doi10.1016/j.chemosphere.2019.124857
dc.identifier.issn0045-6535
dc.identifier.issn1879-1298
dc.identifier.pmid31726599
dc.identifier.scopus2-s2.0-85073110971
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.chemosphere.2019.124857
dc.identifier.urihttps://hdl.handle.net/11480/14570
dc.identifier.volume240
dc.identifier.wosWOS:000497600800068
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofChemosphere
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectColorado potato beetle
dc.subjectDetoxification enzymes
dc.subjectResistance management
dc.subjectSynergism
dc.titleDownregulation of imidacloprid resistant genes alters the biological parameters in Colorado potato beetle, Leptinotarsa decemlineata Say (chrysomelidae: Coleoptera)
dc.typeArticle

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