Chitosan nanoparticles enhances the anti-quorum sensing activity of kaempferol

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dc.authoridIlk, Sedef/0000-0002-6754-792X
dc.authoridSAGLAM, NECDET/0000-0002-5463-8355
dc.contributor.authorIlk, Sedef
dc.contributor.authorSaglam, Necdet
dc.contributor.authorOzgen, Mustafa
dc.contributor.authorKorkusuz, Feza
dc.date.accessioned2024-11-07T13:32:48Z
dc.date.available2024-11-07T13:32:48Z
dc.date.issued2017
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractQuorum sensing (QS) is a cell density dependent expression of species in bacteria mediated by compounds called autoinducers (AI). Several processes responsible for successful establishment of bacterial infection are mediated by QS. Inhibition of QS is therefore being considered as a new target for antimicrobial chemotherapy. Flavonoid compounds are strong antioxidant and antimicrobial agents but their applications are limited due to their poor dissolution and bioavailability. Our objective was to investigate the effect of kaempferol loaded chitosan nanoparticles on modulating QS mediated by AI in model bioassay test systems. For this purpose, kaempferol loaded nanoparticles were synthesized and characterized in terms of hydrodynamic diameter, hydrogen bonding, amorphous transformation and antioxidant activity. QS inhibition in time dependent manner of nanoparticles was measured in violacein pigment producing using the biosensor strain Chromobacterium violaceum CV026 mediated by AI known as acylated homoserine lactone (AHL). Our results indicated that the average kaempferol loaded chitosan/TPP nanoparticle size and zeta potential were 192.27 +/- 13.6 nm and +35 mV, respectively. The loading and encapsulation efficiency of kaempferol into chitosan/TPP nanoparticles presented higher values between 78 and 93%. Kaempferol loaded chitosan/TPP nanoparticle during the 30 storage days significantly inhibited the production of violacein pigment in Chromobacterium violaceum CV026. The observation that kaempferol encapsulated chitosan nanoparticles can inhibit QS related processes opens up an exciting new strategy for antimicrobial chemotherapy as stable QS-based anti-biofilm agents. (C) 2016 Elsevier B.V. All rights reserved.
dc.description.sponsorshipAyhan Sahenk Foundation; Hacettepe University [014 D12 101 005-820]; Scientific and Technological Research Council of Turkey (TUBITAK) [2211/C]
dc.description.sponsorshipWe would like to thank Ayhan Sahenk Foundation for their support for laboratories of Ayhan Sahenk Agricultural Sciences and Technologies Faculty. We are also thankful for Prof. Dr. Robert Mclean from Texas State University, San Marcos for supplying us Chromobacterium violaceum CV026. This work was supported by the research program of the Hacettepe University (014 D12 101 005-820) and The Scientific and Technological Research Council of Turkey (TUBITAK) with PhD Scholarship (2211/C).
dc.identifier.doi10.1016/j.ijbiomac.2016.10.068
dc.identifier.endpage662
dc.identifier.issn0141-8130
dc.identifier.issn1879-0003
dc.identifier.pmid27777079
dc.identifier.scopus2-s2.0-84992488701
dc.identifier.scopusqualityQ1
dc.identifier.startpage653
dc.identifier.urihttps://doi.org/10.1016/j.ijbiomac.2016.10.068
dc.identifier.urihttps://hdl.handle.net/11480/15625
dc.identifier.volume94
dc.identifier.wosWOS:000390621900073
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectAnti-quorum sensing
dc.subjectBiofilm formation
dc.subjectKaempferol
dc.subjectChitosan nanoparticle
dc.subjectChromobacterium violaceum
dc.titleChitosan nanoparticles enhances the anti-quorum sensing activity of kaempferol
dc.typeArticle

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