The effectiveness of antimicrobial photodynamic therapy on catheter infection model

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dc.contributor.authorAysert-Yildiz, Pinar
dc.contributor.authorKalkanci, Ayse
dc.contributor.authorErdogan, Merve
dc.contributor.authorOzger, Hasan Selcuk
dc.contributor.authorOztuerk, Ali
dc.contributor.authorGuezel-Tunccan, Ozlem
dc.contributor.authorDizbay, Murat
dc.date.accessioned2024-11-07T13:23:55Z
dc.date.available2024-11-07T13:23:55Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description2nd Balkan Conference on Medical Mycology and Mycotoxicology -- OCT 06-08, 2022 -- Thessaloniki, GREECE
dc.description.abstractBackground/Aim: This experimental study aimed to examine the effectiveness of transdermal antimicrobial photodynamic therapy (APDT) with and without antimicrobial lock therapy (ALT), on catheter biofilms. Methods: S. epidermidis and C. orthopsilosis biofilms were formed within peripheral venous catheters positioned in the marginal ear veins of New Zealand white rabbits. Biofilm formation was confirmed with scanning electron microscopy in two catheters. 24 catheters with staphylococcal biofilms and 24 with fungal biofilms were treated with APDT, ALT or APDT plus ALT for five days. Six catheters were separated as controls. APDT was applied with a red colored LED lamp and methylene blue as the photosensitizer. Vancomycin lock solutions were used as ALT for staphylococcal biofilms and amphotericin B for fungal biofilms. The effect of treatment procedures was evaluated by intraluminal biofilm viability testing based on spectrophotometric evaluation, and a quantitative (OD) value was obtained for each catheter. Results: The mean OD values obtained by 600 nm spectrophotometric reading at 24 h (biofilm viability) after ALT, APDT and ALT plus APDT procedures were 0.363, 0.151 and 0.128 for S. epidermidis and 0.092, 0.104 and 0.227 for C. orthopsilosis, respectively. All these OD values obtained after treatment procedures were lower than controls for both S. epidermidis (OD: 0,802) and C. orthopsilosis (OD: 0,315), although there were large fluctuations in our results. Conclusions: Our results suggest that transdermal APDT may be an effective method for treating staphylococcal and candida biofilms formed within intravenous catheters in our rabbit ear model. The combined use of APDT and ALT might be beneficial in these staphylococcal biofilms.
dc.description.sponsorshipGazi University Scientific Research Projects Coordination Unit [TCD-2021-7005]
dc.description.sponsorshipThis project was financially supported by Gazi University Scientific Research Projects Coordination Unit with project no: TCD-2021-7005.
dc.identifier.doi10.1016/j.jphotobiol.2024.113026
dc.identifier.issn1011-1344
dc.identifier.issn1873-2682
dc.identifier.pmid39243746
dc.identifier.scopus2-s2.0-85203025352
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.jphotobiol.2024.113026
dc.identifier.urihttps://hdl.handle.net/11480/13809
dc.identifier.volume260
dc.identifier.wosWOS:001310143900001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofJournal of Photochemistry and Photobiology B-Biology
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectCandida
dc.subjectCatheter model
dc.subjectLock therapy
dc.subjectPhotodynamic therapy
dc.subjectStaphylococcus
dc.titleThe effectiveness of antimicrobial photodynamic therapy on catheter infection model
dc.typeConference Object

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