Transgenic potato lines expressing hairpin RNAi construct of molting-associated EcR gene exhibit enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata, Say)

Basit Öğe Kaydı
dc.contributor.authorHussain T.
dc.contributor.authorAksoy E.
dc.contributor.authorÇalışkan M.E.
dc.contributor.authorBakhsh A.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2019
dc.departmentNiğde ÖHÜ
dc.description.abstractMost of the commercialized insect resistant transgenic crops express cry gene(s) isolated from Bacillus thuringiensis; however, intensive cultivation of Bt crops over almost two decades has been questioned regarding its sustainability and durability in pest management. The present study focused on silencing of highly specific molting-associated Ecdysone receptor (EcR) gene of Colorado potato beetle (CPB) using RNA interference (RNAi) approach. The partial cDNA of EcR gene of CPB was amplified using specific primers in sense and anti-sense orientations, and cloned in pRNAi-GG vector flanked by an intronic sequence (pdk). Leaf and internodal explants of Agria and Lady Olympia potato cultivars were infected with Agrobacterium strain LBA4404 harboring constructs under the control of CaMV 35S promoter. Standard molecular analysis of primary transformants showed proper integration of T-DNA in plant genome. The transgenic plants of both cultivars were evaluated for their efficacy against first, second and third instar CPB larvae. The leaf biotoxicity assays revealed 15–80% of CPB mortality. A significantly lower fold-change (0.87–4.14×) in larval weight was observed in insects fed on transgenic plants compared to the ones fed on control plants (1.87–6.53×). Furthermore, CPB larvae fed on transgenic plants exhibited reduced EcR transcripts, indicating the functionality of dsRNA EcR in silencing EcR gene expression. This study is an excellent example of the integration of an alternative, effective and reliable method to cope with potato insect pests that incur significant losses to potato production in the world. © 2019, Springer Nature Switzerland AG.
dc.description.sponsorshipAcknowledgements The present research project was supported by ‘‘The Scientific and Technological Council of Turkey’’ (Tübitak, Project No. 215O520). The authors are highly thankful to Tübitak for providing PhD fellowship to Ms. Tahira Hussain from the project budget. Authors acknowledge the contributions of Prof. Dr. Ayhan GÖKC¸ E, Muhammad Saleem and Mr. Nadir Naqqash for allowing us to use facilities of entomology laboratory in Department of Plant Production and Technologies.
dc.identifier.doi10.1007/s11248-018-0109-7
dc.identifier.endpage164
dc.identifier.issn0962-8819
dc.identifier.issue1
dc.identifier.pmid30607744
dc.identifier.scopus2-s2.0-85059671922
dc.identifier.scopusqualityQ2
dc.identifier.startpage151
dc.identifier.urihttps://dx.doi.org/10.1007/s11248-018-0109-7
dc.identifier.urihttps://hdl.handle.net/11480/1517
dc.identifier.volume28
dc.identifier.wosWOS:000457330600012
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherSpringer International Publishing
dc.relation.ispartofTransgenic Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectEcdysone receptor
dc.subjectInsect resistance
dc.subjectMolting
dc.subjectTransgenic technology
dc.titleTransgenic potato lines expressing hairpin RNAi construct of molting-associated EcR gene exhibit enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata, Say)
dc.typeArticle

Dosyalar

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu