Characterization of bacterial knot disease caused by Pseudomonas savastanoi pv. savastanoi on pomegranate (Punica granatum L.) trees: a new host of the pathogen

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dc.authorid0000-0002-5651-6597
dc.authorid0000-0001-5104-0983
dc.contributor.authorBozkurt, I. A.
dc.contributor.authorSoylu, S.
dc.contributor.authorMirik, M.
dc.contributor.authorSerce, C. Ulubas
dc.contributor.authorBaysal, O.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2014
dc.departmentNiğde ÖHÜ
dc.description.abstractThis study aimed to isolate and identify the causal organism causing hyperplastic outgrowths (knots) on stems and branches of pomegranate trees in the Eastern Mediterranean region of Turkey. Bacterial colonies were isolated from young knots on plates containing selective nutrient media. Biochemical tests, fatty acid analysis and PCR were performed to identify possible causal disease agent. Representative isolates were identified as Pseudomonas.pv.savastanoi (Psv) using biochemical tests, fatty acid profiling and PCR. Following inoculation of pomegranate plants (cv. hicaz) with bacterial suspensions, 25 of 54 bacterial isolates caused typical knots at the site of inoculation. PCR analysis, using specific primer for Psv, generated a single amplicon from all isolates. The similarity of the sequence of Turkish pomegranate isolate was 99% similar to the corresponding gene sequences of Psv in the databases. Based on symptoms, biochemical, molecular, pathogenicity tests and sequence analyses, the disease agent of knots observed on the pomegranate trees is Psv. To the best of our knowledge, this research has revealed pomegranate as a natural host of Psv, which extends the list of host plant species affected by the pathogen in the world and Turkey. Significance and Impact of the StudyPomegranate trees were affected by the disease with outgrowths (galls or knot) disease. Currently, there is no published study on disease agent(s) causing the galls or knots on pomegranate trees in worldwide. Bacterial colonies were isolated from young knots. The causal agent of the knot Pseudomonas savastanoi pv.savastanoi (Psv) was identified based on symptoms, biochemical, molecular methods, pathogenicity tests and sequence analysis. To the best of our knowledge, this is the first report of Psv on pomegranate as a natural host, which extends the growing list of plant species affected by this bacterium in the world and Turkey.
dc.identifier.doi10.1111/lam.12309
dc.identifier.endpage527
dc.identifier.issn0266-8254
dc.identifier.issn1472-765X
dc.identifier.issue5
dc.identifier.pmid25039423
dc.identifier.scopus2-s2.0-84908006562
dc.identifier.scopusqualityQ3
dc.identifier.startpage520
dc.identifier.urihttps://dx.doi.org/10.1111/lam.12309
dc.identifier.urihttps://hdl.handle.net/11480/4109
dc.identifier.volume59
dc.identifier.wosWOS:000343871400010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherWILEY-BLACKWELL
dc.relation.ispartofLETTERS IN APPLIED MICROBIOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectbacterial knot
dc.subjectPCR
dc.subjectpomegranate
dc.subjectPseudomonas savastanoi pv
dc.subjectsavastanoi
dc.subjectTurkey
dc.titleCharacterization of bacterial knot disease caused by Pseudomonas savastanoi pv. savastanoi on pomegranate (Punica granatum L.) trees: a new host of the pathogen
dc.typeArticle

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