DNA based iPBS-retrotransposon markers for investigating the population structure of pea (Pisum Sativum) germplasm from Turkey

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dc.authoridYildiz, Mehtap/0000-0001-6534-5286
dc.authoridSaenz de Miera, Luis E./0000-0003-1871-7379
dc.authoridBALOCH, Faheem Shehzad/0000-0002-7470-0080
dc.authoridOzkan, Hakan/0000-0003-3530-2626
dc.authoridALSALEH, Ahmad/0000-0001-7078-3221
dc.contributor.authorBaloch, Faheem Shehzad
dc.contributor.authorAlsaleh, Ahmad
dc.contributor.authorSaenz de Miera, Luis E.
dc.contributor.authorHatipoglu, Rustu
dc.contributor.authorCiftci, Vandettin
dc.contributor.authorKarakoy, Tolga
dc.contributor.authorYildiz, Mehtap
dc.date.accessioned2024-11-07T13:35:15Z
dc.date.available2024-11-07T13:35:15Z
dc.date.issued2015
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractRetrotransposons have been highly studied in monocots; however retrotransposon diversity in dicot crops has not been well documented. Our objective was to assess the diversity harbored by field pea landraces using retrotranposon markers. In this research, molecular characterization of 104 landraces and 34 field pea breeding lines was assessed using newly developed iPBS-retrotransposon markers. The 12 iPBS-retrotransposon primers generated a total 106 scorable bands, and 81 of these were found to be polymorphic (76.4%), with an average of 6.75 polymorphic fragments per primer. Polymorphism information content (PIC) ranged from 0.33 to 0.84 with an average of 0.61. It was evident that field pea landraces from the same geographical region were often placed in different groups in the neighbor joining analysis, indicating that grouping based on genetic parameters was not closely related to the geographical origin. The population structure was determined by using STRUCTURE software, and three populations at K = 3 and five populations at K = 5 were identified among landraces. The plentiful diversity present in Turkish field pea landraces could be used as genetic resource in designing breeding program, and may also contribute to worldwide pea breeding programs. Our data also suggested a role of iPBS-retrotransposons as 'a universal marker' for molecular characterization of pea germplasm. (C) 2015 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipNigde University, Scientific Research Projects Unit [FEB2013119]
dc.description.sponsorshipWe thank to ICARDA (International Center of Agricultural Research in Dry Areas, Aleppo, Syria) gene bank for the kind provision of landrace seed stocks. The authors express their gratitude to Nigde University, Scientific Research Projects Unit (FEB2013119) for their financial support.
dc.identifier.doi10.1016/j.bse.2015.06.017
dc.identifier.endpage252
dc.identifier.issn0305-1978
dc.identifier.issn1873-2925
dc.identifier.scopus2-s2.0-84932604218
dc.identifier.scopusqualityQ3
dc.identifier.startpage244
dc.identifier.urihttps://doi.org/10.1016/j.bse.2015.06.017
dc.identifier.urihttps://hdl.handle.net/11480/16413
dc.identifier.volume61
dc.identifier.wosWOS:000361164800033
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofBiochemical Systematics and Ecology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectDiversity
dc.subjectGenetic resources
dc.subjectLandraces
dc.subjectPea
dc.subjectRetrotransposons
dc.subjectTurkey
dc.titleDNA based iPBS-retrotransposon markers for investigating the population structure of pea (Pisum Sativum) germplasm from Turkey
dc.typeArticle

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