Enzyme assay techniques and protocols

Küçük Resim Yok

Tarih

2022

Dergi Başlığı

Dergi ISSN

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Yayıncı

Elsevier

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Enzyme assays are standardized experimental protocols, which are established in order to measure the activity or concentration of enzymes in biochemical or cell-based systems. Mostly enzymatic assays are based upon the detection of fluorescent, luminescent, or spectrophotometric endpoint signal. During recent years, florescence-based, absorbance-based, and luminescence-based high-throughput screening assays have been developed and widely used in various fields. Enzyme assays represent an important tool for screening of sample libraries in context of drug discovery. In addition, enzyme assays are valuable for diagnosis of several diseases in clinical settings due to their high sensitivity, specificity, and rapid response. In this chapter, we aim to provide an understanding of the working principle of enzymatic assays and their applications with some specific examples of enzyme assays that are used for drug discovery and diagnostic purpose. © 2022 Elsevier Inc. All rights reserved.

Açıklama

Anahtar Kelimeler

disease diagnosis, drug discovery, Enzymes, high-throughput screening

Kaynak

Analytical Techniques in Biosciences: from Basics to Applications

WoS Q Değeri

Scopus Q Değeri

N/A

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