In vitro and in vivo investigation of Argiope bruennichi spider silk-based novel biomaterial for medical use

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dc.contributor.authorTuran, Secil Karahisar
dc.contributor.authorSuloglu, Aysun Kilic
dc.contributor.authorIde, Semra
dc.contributor.authorTurkes, Tuncay
dc.contributor.authorBarlas, Nurhayat
dc.date.accessioned2024-11-07T13:34:30Z
dc.date.available2024-11-07T13:34:30Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractAs a natural and biocompatible material with high strength and flexibility, spider silk is frequently used in biomedical studies. In this study, the availability of Argiope bruennichi spider silk as a surgical suture material was investigated. The effects of spider silk-based and commercial sutures, with and without Aloe vera coating, on wound healing were evaluated by a rat dorsal skin flap model, postoperatively (7th and 14th days). Biochemical, hematological, histological, immunohistochemical, small angle x-ray scattering (SAXS) analyses and mechanical tests were performed. A. bruennichi silk did not show any cytotoxic effect on the L929 cell line according to MTT and LDH assays, in vitro. The silk materials did not cause any allergic reaction, infection, or systemic effect in rats according to hematological and biochemical analyses. A. bruennichi spider silk group showed a similar healing response to commercial sutures. SAXS analysis showed that the 14th-day applications of A. bruennichi spider silk and A. vera coated commercial suture groups have comparable structural results with control group. In conclusion, A. bruennichi spider silk is biocompatible in line with the parameters examined and shows a healing response similar to the commercial sutures commonly used in the skin.
dc.description.sponsorshipHacettepe University Scientific Research Projects Coordination; [FBA-2018-16615]
dc.description.sponsorshipThis research is supported by Hacettepe University Scientific Research Projects Coordination (Grant No: FBA-2018-16615).
dc.identifier.doi10.1002/bip.23572
dc.identifier.issn0006-3525
dc.identifier.issn1097-0282
dc.identifier.issue3
dc.identifier.pmid38491802
dc.identifier.scopus2-s2.0-85188465188
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1002/bip.23572
dc.identifier.urihttps://hdl.handle.net/11480/16018
dc.identifier.volume115
dc.identifier.wosWOS:001185869200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiopolymers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectAloe vera
dc.subjectArgiope bruennichi
dc.subjecthistology
dc.subjectSAXS analysis
dc.subjectspider silk
dc.subjectwound healing
dc.titleIn vitro and in vivo investigation of Argiope bruennichi spider silk-based novel biomaterial for medical use
dc.typeArticle

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