L-Arginine Inhibits Apoptosis of Ovine Intestinal Epithelial Cells through the L-Arginine-Nitric Oxide Pathway

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dc.authoridWang, Hongrong/0000-0002-4980-1082
dc.authoridElsabagh, Mabrouk/0000-0002-0596-6547
dc.contributor.authorZhang, Hao
dc.contributor.authorZhao, Fangfang
dc.contributor.authorPeng, Along
dc.contributor.authorGuo, Shuang
dc.contributor.authorWang, Mengzhi
dc.contributor.authorElsabagh, Mabrouk
dc.contributor.authorLoor, Juan J.
dc.date.accessioned2024-11-07T13:35:12Z
dc.date.available2024-11-07T13:35:12Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground: In nonruminants, many of the biological roles of L-arginine (Arg) at the intestinal level are mediated through the Arg-nitric oxide (Arg-NO) pathway. Whether the Arg-NO pathway is involved in controlling the immune response and viability in ovine intestinal epithelial cells (IOECs) is unclear. Objectives: The current study aimed to examine the role of the Arg-NO pathway in apoptosis, antioxidant capacity, and mitochondrial function of IOECs. Methods: The IOECs were incubated in Arg-free DMEM supplemented with 150 mu M Arg (CON) or 300 mu M Arg (ARG) alone or with 350 mu M Nw-nitro-L-arginine methyl ester hydrochloride (L-NAME) (CON + NAME, ARG + NAME) for 24 h. The reactive oxygen species (ROS) concentration, antioxidant capacity, and cell apoptotic percentage were determined. Results: Arg supplementation decreased (P < 0.05) the ROS concentration (38.9% and 22.7%) and apoptotic cell percentage (57.2% and 54.8%) relative to the CON and CON + NAME groups, respectively. Relative to the CON and ARG treatments, the L-NAME administration decreased (P < 0.05) the mRNA abundance of superoxide dismutase 2 (32% and 21.3%, respectively) and epithelial NO synthase (36% and 29.1%, respectively). Arg supplementation decreased (P < 0.05) the protein abundance of apoptosis antigen 1 (FAS) (52.0% and 43.9%) but increased (P < 0.05) those of nuclear respiratory factor 1 (31.3% and 22.9%) and inducible NO synthase (35.2% and 41.8%) relative to the CON and CON + NAME groups, respectively. Conclusions: The inhibition of apoptosis in IOECs due to the increased supply of Arg is associated with the mitochondria- and FAS-dependent pathways through the activity of the Arg-NO pathway. The findings help elucidate the role of the Arg-NO pathway in IOEC growth and apoptosis.
dc.description.sponsorshipNational Natural Science Foundation of China [31902180]; Natural Science Foundation of Jiangsu Province Research Project [BK20170488]; China Postdoctoral Science Foundation [2017M610358]
dc.description.sponsorshipSupported by National Natural Science Foundation of China grant 31902180, Natural Science Foundation of Jiangsu Province Research Project grant BK20170488, and China Postdoctoral Science Foundation grant 2017M610358.
dc.identifier.doi10.1093/jn/nxaa133
dc.identifier.endpage2060
dc.identifier.issn0022-3166
dc.identifier.issn1541-6100
dc.identifier.issue8
dc.identifier.pmid32412630
dc.identifier.scopus2-s2.0-85089127057
dc.identifier.scopusqualityQ1
dc.identifier.startpage2051
dc.identifier.urihttps://doi.org/10.1093/jn/nxaa133
dc.identifier.urihttps://hdl.handle.net/11480/16364
dc.identifier.volume150
dc.identifier.wosWOS:000555973600010
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherOxford Univ Press
dc.relation.ispartofJournal of Nutrition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectL-arginine
dc.subjectapoptosis
dc.subjectantioxidant
dc.subjectmitochondrial function
dc.subjectArg-NO pathway
dc.titleL-Arginine Inhibits Apoptosis of Ovine Intestinal Epithelial Cells through the L-Arginine-Nitric Oxide Pathway
dc.typeArticle

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