Effects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats

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dc.contributor.authorKoksal, M.
dc.contributor.authorOguz, E.
dc.contributor.authorBaba, E.
dc.contributor.authorEren, M. Ali
dc.contributor.authorCiftci, H.
dc.contributor.authorDemir, M. E.
dc.contributor.authorUlas, T.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2012
dc.departmentNiğde ÖHÜ
dc.description.abstractBackground: Testicular torsion due to oxidative stress results in infertility and testicular damage which can be preventable an important health problem worldwide. Aim: The purpose of the present study was to investigate the changes of malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels; histopathological alterations; morphology, concentration and motilities of the sperm in post ischemic reperfused (I/R) testis tissue. Materials and Methods: Forty adult male Wistar rats were carried out and were randomized to five groups; (1) Control group, (2) Ipsilateral left testis ischemia, (3) Melatonin plus ipsilateral left testis ischemia, (4) Contra lateral right testis ischemia, 5. Melatonin plus contralateral right testis ischemia. After 1 h ischemia and 24 h perfusion; MDA, TAS and TOS levels were measured, histopathological alterations were determined using by Johnsen's score (JS) and sperm morphology, concentration, motility were examined. Results: MDA,TAS and TOS levels of the testis tissue did not change in all groups (p > 0.05 for all). JS was decreased in I/R group and melatonin treatment reversed histopathological changes and increased JS both in ipsilateral and contralateral testis. Abnormal sperm rate significantly increased in I/R group and melatonin administration changed abnormal sperm rate to normal. Conclusions: As a result, the present study demonstrated that testicular damage occurs following I/R without an increase of MDA, TAS and TOS levels. Our results also suggested that melatonin is a potent antioxidant agent in preventing testicular I/R injury, as shown by increased JS and changed abnormal sperm rate.
dc.description.sponsorshipScientific and Technical Research Council of Turkey (Tubitak) [SBAG- 104S257]
dc.description.sponsorshipThis study was supported by a grant from The Scientific and Technical Research Council of Turkey (Tubitak) (SBAG- 104S257).
dc.identifier.endpage588
dc.identifier.issn1128-3602
dc.identifier.issue5
dc.identifier.pmid22774397
dc.identifier.scopus2-s2.0-84864082535
dc.identifier.scopusqualityQ2
dc.identifier.startpage582
dc.identifier.urihttps://hdl.handle.net/11480/4579
dc.identifier.volume16
dc.identifier.wosWOS:000305663400003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherVERDUCI PUBLISHER
dc.relation.ispartofEUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIschemia-reperfusion
dc.subjectMelatonin
dc.subjectTestis
dc.subjectOxidative stress
dc.subjectJohnsen's score
dc.titleEffects of melatonin on testis histology, oxidative stress and spermatogenesis after experimental testis ischemia-reperfusion in rats
dc.typeArticle

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