Estrogen as a Novel Agent for Induction of Adipose-Derived Mesenchymal Stem Cells for Osteogenic Differentiation: In Vivo Bone Tissue-Engineering Study

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dc.authorid0000-0001-8717-4668
dc.authorid0000-0002-7909-7604
dc.contributor.authorCalis, Mert
dc.contributor.authorDemirtas, Tugrul Tolga
dc.contributor.authorAtilla, Pergin
dc.contributor.authorTatar, Ilkan
dc.contributor.authorErsoy, Orkun
dc.contributor.authorIrmak, Gulseren
dc.contributor.authorOzgur, Figen
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2014
dc.departmentNiğde ÖHÜ
dc.description.abstractBackground: This study investigated whether the in vivo osteogenic differentiation potential of adipose-derived mesenchymal stem cells is enhanced by 17-estradiol. Methods: Thirty Sprague-Dawley rats were randomized and divided into five experimental groups. For the surgical procedure, biparietal full-thickness bone defects (7 mm in diameter) were created. A chitosan-hydroxyapatite scaffold was used as the vehicle system for 17-estradiol-loaded nanoparticles and adipose-derived mesenchymal stem cells. The first group, the blank defect group, was the control group. The defects were filled with either scaffold, estradiol, and scaffold; scaffold and adipose-derived mesenchymal stem cells; or estradiol, scaffold, and adipose-derived mesenchymal stem cells as experimental groups. The rats were killed at the end of weeks 4 and 12, and their calvariae were harvested for histologic and microtomographic evaluation. Results: Micro-computed tomographic evaluation of estradiol, scaffold, and adipose-derived mesenchymal stem cells revealed the highest median value (82.59 17.17), and the difference was significant compared with the blank defect group (p = 0.004). Histologic samples demonstrated a significant difference between experimental groups for bone defect repair at the end of weeks 4 and 12 (p = 0.003 and p < 0.001). The estradiol, scaffold, and adipose-derived mesenchymal stem cell group had the highest median score (3.00 +/- 0.0) at week 12, which was significantly higher than scores for the scaffold and adipose-derived mesenchymal stem cell group and the blank defect group. Conclusion: 17-Estradiol appears to be a novel and promising agent for future cell-based bone tissue-engineering studies.
dc.identifier.doi10.1097/PRS.0000000000000056
dc.identifier.endpage510E
dc.identifier.issn0032-1052
dc.identifier.issn1529-4242
dc.identifier.issue4
dc.identifier.pmid24675202
dc.identifier.startpage499E
dc.identifier.urihttps://dx.doi.org/10.1097/PRS.0000000000000056
dc.identifier.urihttps://hdl.handle.net/11480/4182
dc.identifier.volume133
dc.identifier.wosWOS:000335988600007
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherLIPPINCOTT WILLIAMS & WILKINS
dc.relation.ispartofPLASTIC AND RECONSTRUCTIVE SURGERY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleEstrogen as a Novel Agent for Induction of Adipose-Derived Mesenchymal Stem Cells for Osteogenic Differentiation: In Vivo Bone Tissue-Engineering Study
dc.typeArticle

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