Investigation of the potential teratogenic effects of fructose on the embryo using the rat whole embryo culture model

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dc.contributor.authorGür, Hatice Emel
dc.contributor.authorBalcıoğlu, Esra
dc.contributor.authorPatat, Dilara
dc.contributor.authorUçar, Sümeyye
dc.contributor.authorGür, Fatih Mehmet
dc.contributor.authorYalçın, Betül
dc.contributor.authorNisari, Mehtap
dc.date.accessioned2024-11-07T10:39:38Z
dc.date.available2024-11-07T10:39:38Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractExcessive consumption of fructose-sweetened foods and beverages is a growing concern worldwide. Studies have demonstrated that fructose consumption before and during pregnancy can result in adverse outcomes such as decreased decidualization, increased fetal losses, and low birth weight. The study investigated the teratogenic effects of fructose on rat embryos during organogenesis using whole embryo culture. Within the scope of the study, 4 groups were formed as control, low, medium, and high-dose fructose (HDF) with 10 embryos in each group. The 9.5-day-old rat embryos were cultured with different concentrations of fructose (1, 5 and 10 mM) for 48 h and the possible effects of fructose were examined using morphological scoring, histochemistry, immunofluorescence, and TUNEL methods. According to the analyses, protein synthesis and proliferation were decreased, vascular formation was suppressed, and apoptosis was increased in embryos exposed to fructose, especially at concentrations of 5 mM and above. According to the morphological scoring results, it was determined that heart, hind limb, and somite development were retarded in all experimental groups compared to the control group, developmental retardation increased in direct proportion to fructose concentration, and also significant malformations were observed in all parameters examined in the HDF group. In addition, analysis of yolk sac diameter, head length, crown rump length and somite numbers showed that these parameters were significantly decreased in all experimental groups. End of the study, it was concluded that fructose at concentrations of 1 mM and above may induce embryonic development retardation and other anomalies by decreasing protein synthesis and cell proliferation, suppressing vascular formation, and increasing apoptosis in embryonic tissues. © 2024 Elsevier Ltd
dc.description.sponsorshipTurkey Projects Commission; Erciyes University Faculty of Medicine Hakan Çetinsaya Experimental Research and Application Center; Erciyes University Scientific Research Kayseri; Erciyes Üniversitesi, (19/218); DEKAM, (17.00, 08.00); Erciyes University Scientific Research Projects Commission, (TYL-2020-10011)
dc.identifier.doi10.1016/j.fct.2024.114985
dc.identifier.issn0278-6915
dc.identifier.scopus2-s2.0-85203652329
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.fct.2024.114985
dc.identifier.urihttps://hdl.handle.net/11480/11097
dc.identifier.volume193
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Ltd
dc.relation.ispartofFood and Chemical Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectApoptosis
dc.subjectEmbryonic development"
dc.subjectGrowth retardation
dc.subjectVascularization
dc.subjectWhole embryo culture
dc.titleInvestigation of the potential teratogenic effects of fructose on the embryo using the rat whole embryo culture model
dc.typeArticle

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