Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd

dc.authoridTek, Ahmet L./0000-0002-3292-5142
dc.contributor.authorTek, Ahmet L.
dc.contributor.authorYildiz, Humeyra
dc.contributor.authorKhan, Kamran
dc.contributor.authorYildirim, Bilge S.
dc.date.accessioned2024-11-07T13:24:34Z
dc.date.available2024-11-07T13:24:34Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractRibosomal DNAs and various banding patterns are landmarks in molecular cytogenetics providing useful information for karyotyping and addressing individual chromosomes. Bottle gourd is the only cultivated species of the Lagenaria genus with high genetic diversity. After CMA(3)/DAPI fluorochrome banding we investigated the GC- and AT-rich regions in interphase nuclei of five different local accessions. Fluorescence in situ hybridization (FISH) was conducted to determine the number and location of 45S and 5S rDNAs in bottle gourd. Our results showed four strong CMA(3) regions in interphase and on mitotic metaphase chromosomes. FISH revealed four strong signals of 45S rDNA at the termini of two metaphase chromosome pairs and terminal 5S rDNA signals at another pair of chromosomes. The presence of four positive CMA(3) bands colocalizes with four 45S rDNA signals in all bottle gourd accessions. Our results allow distinguishing two out of eleven chromosome pairs of bottle gourd.
dc.description.sponsorshipINDEPTH-COST Action [CA16212]; Ayhan Sahenk Foundation; YOK 100/2000 Plant Genetics and Agricultural Biotechnology
dc.description.sponsorshipThis work was partially supported by the INDEPTH-COST Action CA16212 and scholarship program from Ayhan Sahenk Foundation. HY and BSY recognize the scholarship from YOK 100/2000 Plant Genetics and Agricultural Biotechnology.
dc.identifier.doi10.36253/caryologia-1134
dc.identifier.endpage8
dc.identifier.issn0008-7114
dc.identifier.issn2165-5391
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85125233193
dc.identifier.scopusqualityQ2
dc.identifier.startpage3
dc.identifier.urihttps://doi.org/10.36253/caryologia-1134
dc.identifier.urihttps://hdl.handle.net/11480/14176
dc.identifier.volume74
dc.identifier.wosWOS:000753456500001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherFirenze Univ Press
dc.relation.ispartofCaryologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectbottle gourd
dc.subjectchromomycin A3
dc.subjectfluorescence in situ hybridization
dc.subjectlocal accessions
dc.subjectribosomal DNA
dc.subject5S
dc.subject45S
dc.titleChromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
dc.typeArticle

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