Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
dc.authorid | Tek, Ahmet L./0000-0002-3292-5142 | |
dc.contributor.author | Tek, Ahmet L. | |
dc.contributor.author | Yildiz, Humeyra | |
dc.contributor.author | Khan, Kamran | |
dc.contributor.author | Yildirim, Bilge S. | |
dc.date.accessioned | 2024-11-07T13:24:34Z | |
dc.date.available | 2024-11-07T13:24:34Z | |
dc.date.issued | 2021 | |
dc.department | Niğde Ömer Halisdemir Üniversitesi | |
dc.description.abstract | Ribosomal DNAs and various banding patterns are landmarks in molecular cytogenetics providing useful information for karyotyping and addressing individual chromosomes. Bottle gourd is the only cultivated species of the Lagenaria genus with high genetic diversity. After CMA(3)/DAPI fluorochrome banding we investigated the GC- and AT-rich regions in interphase nuclei of five different local accessions. Fluorescence in situ hybridization (FISH) was conducted to determine the number and location of 45S and 5S rDNAs in bottle gourd. Our results showed four strong CMA(3) regions in interphase and on mitotic metaphase chromosomes. FISH revealed four strong signals of 45S rDNA at the termini of two metaphase chromosome pairs and terminal 5S rDNA signals at another pair of chromosomes. The presence of four positive CMA(3) bands colocalizes with four 45S rDNA signals in all bottle gourd accessions. Our results allow distinguishing two out of eleven chromosome pairs of bottle gourd. | |
dc.description.sponsorship | INDEPTH-COST Action [CA16212]; Ayhan Sahenk Foundation; YOK 100/2000 Plant Genetics and Agricultural Biotechnology | |
dc.description.sponsorship | This work was partially supported by the INDEPTH-COST Action CA16212 and scholarship program from Ayhan Sahenk Foundation. HY and BSY recognize the scholarship from YOK 100/2000 Plant Genetics and Agricultural Biotechnology. | |
dc.identifier.doi | 10.36253/caryologia-1134 | |
dc.identifier.endpage | 8 | |
dc.identifier.issn | 0008-7114 | |
dc.identifier.issn | 2165-5391 | |
dc.identifier.issue | 3 | |
dc.identifier.scopus | 2-s2.0-85125233193 | |
dc.identifier.scopusquality | Q2 | |
dc.identifier.startpage | 3 | |
dc.identifier.uri | https://doi.org/10.36253/caryologia-1134 | |
dc.identifier.uri | https://hdl.handle.net/11480/14176 | |
dc.identifier.volume | 74 | |
dc.identifier.wos | WOS:000753456500001 | |
dc.identifier.wosquality | Q4 | |
dc.indekslendigikaynak | Web of Science | |
dc.indekslendigikaynak | Scopus | |
dc.language.iso | en | |
dc.publisher | Firenze Univ Press | |
dc.relation.ispartof | Caryologia | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.snmz | KA_20241106 | |
dc.subject | bottle gourd | |
dc.subject | chromomycin A3 | |
dc.subject | fluorescence in situ hybridization | |
dc.subject | local accessions | |
dc.subject | ribosomal DNA | |
dc.subject | 5S | |
dc.subject | 45S | |
dc.title | Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd | |
dc.type | Article |