Differential effects of resolvin D1 and resolvin E1 on cementoblast function

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dc.authoridBozkurt, Serife Buket/0000-0002-8641-2844
dc.authoridHakki, Sema/0000-0001-8665-6235
dc.contributor.authorBozkurt, Serife Buket
dc.contributor.authorHakki, Sema Sezgin
dc.contributor.authorKantarci, Alpdogan
dc.date.accessioned2024-11-07T13:34:23Z
dc.date.available2024-11-07T13:34:23Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackgroundResolvins are endogenous mediators of the resolution of inflammation. They are derived from omega-3 polyunsaturated fatty acid precursors. Resolvin D1 (RvD1) and Resolvin E1 (RvE1) are the best-characterized members for actively promoting periodontal regeneration in experimental animal models. Here, we evaluated the efficacy of RvD1 and RvE1 on cementoblasts, the key cells involved in dental cementum regeneration and the attachment of the tooth to the alveolar bone. MethodsImmortalized mouse cementoblasts (OCCM-30) were treated with different concentrations (0.1-1000 ng/mL) of RvD1 and RvE1. Cell proliferation was measured using an electrical impedance-based real-time cell analyzer. Mineralization was evaluated with von Kossa staining. The mRNA expression of mineralized tissue-associated markers of bone sialoprotein (BSP), Type I collagen (COL I), osteocalcin (OCN), osteopontin (OPN), runt-related transcription factor 2 (RunX2), alkaline phosphatase (ALP), osteoprotegerin (OPG), receptor activator of nuclear factor kappa B (NF-kappa B) (RANK), receptor activator of NF-kappa B ligand (RANKL), and extracellular matrix-degrading enzymes [matrix metalloproteinase (MMP)-1, MMP-2, MMP-3, MMP-9, and their tissue inhibitors (TIMP-1, TIMP-2)], RvE1 receptor (ChemR23) and RvD1 receptor (ALX/PFR2), cytokines (tumor necrosis factor-alpha {TNF-alpha}, interleukin {IL}-1 beta, IL-6, IL-8, IL-10, IL-17), oxidative stress enzymes [superoxide dismutase (SOD), glutathione peroxidase (GPX), and cyclooxygenase-2 (Cox-2)] were analyzed using quantitative polymerase chain reaction (qPCR). ResultsBoth RvD1 and RvE1 (10-100 ng/mL) significantly increased the proliferation of cementoblasts and mineralized nodules at all concentrations (p < 0.05). RvE1 increased BSP, RunX2, and ALP compared with the RvD1 dose and time-dependently, while RvD1 and RvE1 differentially regulated COL-I. RvE1 increased OPG mRNA expression, whereas RANK-RANKL mRNA expression decreased by RvE1. MMP-2, MMP-3, MMP-9, TIMP-1, and TIMP-2 expressions were reduced by RvE1 compared with RvD1. Treatment of cementoblasts with RvD1 and RvE1 differentially affected cytokine and oxidative stress enzymes while significantly increasing their receptor expressions (ChemR23 and ALX/PFR2). ConclusionsRvD1 and RvE1 regulate proliferation, mineralization, and gene expression in cementoblasts using similar pathways while differentially affecting tissue degradation, suggesting a targeted therapeutic approach for cementum turnover during periodontal regeneration.
dc.description.sponsorshipSelcuk University Coordination of Scientific Research [17401164]
dc.description.sponsorshipACKNOWLEDGMENT This study has been performed with project support from Selcuk University Coordination of Scientific Research (project no 17401164).
dc.identifier.doi10.1002/JPER.22-0510
dc.identifier.endpage1362
dc.identifier.issn0022-3492
dc.identifier.issn1943-3670
dc.identifier.issue11
dc.identifier.pmid37322861
dc.identifier.scopus2-s2.0-85162030480
dc.identifier.scopusqualityQ1
dc.identifier.startpage1351
dc.identifier.urihttps://doi.org/10.1002/JPER.22-0510
dc.identifier.urihttps://hdl.handle.net/11480/15953
dc.identifier.volume94
dc.identifier.wosWOS:001006367000001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Periodontology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectcementum
dc.subjectinflammation
dc.subjectperiodontal regeneration
dc.titleDifferential effects of resolvin D1 and resolvin E1 on cementoblast function
dc.typeArticle

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