Does rhizospheric bacterium influence root exudation of sucrose? A preliminary investigation using Bacillus subtilis RR4
Küçük Resim Yok
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Research Journal Biotechnology
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Root exudation, the secretion of phytochemicals through roots, plays a critical role in regulating the microbial community in the plant rhizosphere. Sugars, particularly sucrose, comprise a major portion among the root exudates. Our aim was to demonstrate the effect of a rice rhizosphere isolate, Bacillus subtilis RR4, on the gene expression of sucrose transporters (OsSUT1-5) and root exudation of sucrose. Aseptically grown rice plants were treated with RR4 in a hydroponic setup for 48 h. Semi-quantitative reverse transcription-PCR was performed using RR4-treated rice roots to analyze the gene expression profile of the sucrose transporters (OsSUT1-5). HPLC analysis of the collected root exudates was performed to analyze the levels of sucrose exuded by the RR4-treated and untreated rice plants. Semi-quantitative RT-PCR analysis of sucrose transporter genes (OsSUT1-5) and HPLC analysis of root exudates revealed that B. subtilis RR4 did not influence the exudation of sucrose.
Açıklama
Anahtar Kelimeler
Bacillus subtilis, root exudation, sucrose, sucrose transporters
Kaynak
Research Journal of Biotechnology
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
13
Sayı
11
