Detection of Escherichia coli O157:H7 using immunomagnetic separation and mPCR in Turkish foods of animal origin

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dc.authorid0000-0003-2721-9275
dc.authorid0000-0002-3935-6296
dc.contributor.authorErtas, N.
dc.contributor.authorGonulalan, Z.
dc.contributor.authorYildirim, Y.
dc.contributor.authorKaradal, F.
dc.contributor.authorAbay, S.
dc.contributor.authorAl, S.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2013
dc.departmentNiğde ÖHÜ
dc.description.abstractThe present study was conducted to investigate the presence of Escherichia coli O157:H7 in food samples of animal origin and to detect its virulence genes by immunomagnetic separation technique and multiplex PCR (mPCR). A total of 500 samples (consisting of diced meat, minced meat, burger, raw cow's milk and raw cow's milk cheese) were analysed. Escherichia coli O157:H7 was detected in 5 (1%) of 500 analysed samples including two diced meat, one minced meat and two raw-milk cheese. None of the burger samples tested contained E.coli O157:H7. Three isolates obtained from minced and diced meat were found to carry stx(1), stx(2), hlyA and eaeA genes whereas two isolates from raw-milk cheese were found to harbour the stx(1), eaeA and hlyA genes. The results of this study suggest that raw meat and raw-milk cheese tested could pose public health problems in consumers with regard to their virulence factors.
dc.identifier.doi10.1111/lam.12124
dc.identifier.endpage379
dc.identifier.issn0266-8254
dc.identifier.issn1472-765X
dc.identifier.issue4
dc.identifier.pmid23809061
dc.identifier.scopus2-s2.0-84884703864
dc.identifier.scopusqualityQ3
dc.identifier.startpage373
dc.identifier.urihttps://dx.doi.org/10.1111/lam.12124
dc.identifier.urihttps://hdl.handle.net/11480/4349
dc.identifier.volume57
dc.identifier.wosWOS:000325034500018
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherWILEY
dc.relation.ispartofLETTERS IN APPLIED MICROBIOLOGY
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectEscherichia coli O157:H7
dc.subjectfoods of animal origin
dc.subjectimmunomagnetic separation
dc.subjectmPCR
dc.subjectvirulence genes
dc.titleDetection of Escherichia coli O157:H7 using immunomagnetic separation and mPCR in Turkish foods of animal origin
dc.typeArticle

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