Towards better insect management strategy: restriction of insecticidal gene expression to biting sites in transgenic cotton

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dc.authorid0000-0001-5110-6014
dc.authorid0000-0003-3561-7863
dc.authorid0000-0002-3091-2639
dc.authorid0000-0002-8524-9029
dc.authorid0000-0002-1697-9327
dc.authorid0000-0002-1697-9327
dc.contributor.authorAnayol, Emine
dc.contributor.authorBakhsh, Allah
dc.contributor.authorKarakoc, Omer Cem
dc.contributor.authorOnarici, Selma
dc.contributor.authorKom, Deniz
dc.contributor.authorAasim, Muhammad
dc.contributor.authorOzcan, Sebahattin
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractMost of the commercialized Bt crops express cry genes under 35S promoter that induces strong gene expression in all plant parts. However, targeted foreign gene expression in plants is esteemed more important as public may be likely to accept 'less intrusive' expression of transgene. We developed plant expression constructs harboring cry1Ac gene under control of wound-inducible promoter (AoPR1) to confine Bt gene expression in insect wounding parts of the plants in comparison with cry1Ac gene under the control of 35S promoter. The constructs were used to transform four Turkish cotton cultivars (GSN-12, STN-468, Ozbek-100 and Ayhan-107) through Agrobacterium tumefaciens strains GV2260 containing binary vectors p35SAcBAR.101 and AoPR1AcBAR.101 harboring cry1Ac gene under control of 35S and AoPR1, respectively. Phosphinothricin (PPT) was used at concentration of 5 mg L-1 for selection of primary transformants. The primary transformants were analyzed for transgene presence and expression standard molecular techniques. The transformants exhibited appreciable mortality rates against larvae of Spodoptera exigua and S. littoralis. It was found that mechanical wounding of T (1) transgenic plants was effective in inducing expression of cry1Ac protein as accumulated levels of cry1Ac protein increased during post-wounding period. We conclude that use of wound-inducible promoter to drive insecticidal gene(s) can be regarded as a valuable insect-resistant management strategy since the promoter activity is limited to insect biting sites of plant. There is no Bt toxin accumulation in unwounded plant organs, seed and crop residues, cotton products and by-products, thus minimizing food and environmental concerns.
dc.description.sponsorshipScientific and Technological Research Council of Turkey TUBITAK [111O254]
dc.description.sponsorshipThe work on development of transgenic cotton in our laboratory is being supported by grants from Scientific and Technological Research Council of Turkey TUBITAK (Project No. 111O254). The authors acknowledge contribution and support of TUBITAK. The authors are also thankful to Leicester University (UK) for giving permission to use AoPR1 promoter for research purposes.
dc.identifier.doi10.1007/s11816-016-0388-5
dc.identifier.endpage94
dc.identifier.issn1863-5466
dc.identifier.issn1863-5474
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84962355440
dc.identifier.scopusqualityQ2
dc.identifier.startpage83
dc.identifier.urihttps://dx.doi.org/10.1007/s11816-016-0388-5
dc.identifier.urihttps://hdl.handle.net/11480/3684
dc.identifier.volume10
dc.identifier.wosWOS:000373886900005
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofPLANT BIOTECHNOLOGY REPORTS
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGenetic modification
dc.subjectInsect resistance
dc.subjectConfined expression
dc.subjectInsect management
dc.titleTowards better insect management strategy: restriction of insecticidal gene expression to biting sites in transgenic cotton
dc.typeArticle

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