Identification and characterization of a novel Robigovirus species from sweet cherry in turkey

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dc.contributor.authorÇağlayan K.
dc.contributor.authorRoumi V.
dc.contributor.authorGazel M.
dc.contributor.authorElçi E.
dc.contributor.authorAcioğlu M.
dc.contributor.authorPlesko I.M.
dc.contributor.authorMassart S.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2019
dc.departmentNiğde ÖHÜ
dc.description.abstractHigh throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5' UTR and 3' UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, RdRp, TGB1, TGB2, TGB3 and CP share amino acid identities ranging from 43–53%, 44–60%, 39–43%, 38–44% and 45–50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.sponsorshipJavna Agencija za Raziskovalno Dejavnost RS
dc.description.sponsorshipFunding: This work was supported by TUBITAK-TOVAG grant number 213O042, the Slovenian Research Agency grant P4-0072 and by the COST Action grant number FA1407 (DIVAS).
dc.identifier.doi10.3390/pathogens8020057
dc.identifier.issn2076-0817
dc.identifier.issue2
dc.identifier.pmid31035571
dc.identifier.scopus2-s2.0-85068536171
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://dx.doi.org/10.3390/pathogens8020057
dc.identifier.urihttps://hdl.handle.net/11480/1477
dc.identifier.volume8
dc.identifier.wosWOS:000475287800016
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherMDPI AG
dc.relation.ispartofPathogens
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBetaflexiviridae
dc.subjectCherry virus Turkey
dc.subjectHigh throughput sequencing
dc.subjectPrunus avium
dc.titleIdentification and characterization of a novel Robigovirus species from sweet cherry in turkey
dc.typeArticle

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