DEVELOPMENT OF INSECT-RESISTANT COTTON LINES WITH TARGETED EXPRESSION OF INSECTICIDAL GENE

dc.authorid0000-0003-3561-7863
dc.authorid0000-0002-1697-9327
dc.authorid0000-0002-1697-9327
dc.contributor.authorBakhsh, Allah
dc.contributor.authorAnayol, Emine
dc.contributor.authorKhabbazi, Saber Delpasand
dc.contributor.authorKarakoc, Omer Cem
dc.contributor.authorSancak, Cengiz
dc.contributor.authorOzcan, Sebahattin
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractIn order to address biosafety concerns regarding the constitutive expression of foreign genes in crops, we applied a strategy aimed at confining foreign gene expression in insect wounding sites of cotton. For this purpose, a plant expression construct was designed by cloning the AoPR1 promoter (pathogenesis-related protein gene isolated from Asparagus officinalis) upstream from the insecticidal gene cry1Ac. The Turkish cotton cultivar cv. STN-468 was transformed using the Agrobacterium tumefaciens strain LBA4404 containing the recombinant binary vector pRD400 harboring cry1Ac under a wound-inducible promoter. The neomycin phosphotransferase (nptII) gene was used as a selectable marker at a concentration of 100 mg/L. The primary transformants were analyzed for T-DNA integration and expression using standard molecular approaches. The efficacy of insecticidal gene control of the AoPR1 promoter was investigated using leaf bioassays with 2nd instar larvae of Helicoverpa armigera and Spodoptera littoralis. Positive primary transformants from T-0 progeny were further raised under greenhouse conditions to obtain progeny (T-1). The introduced gene was properly inherited and expressed in T-1 progeny. The mechanical wounding of plants resulted in increased cry1Ac protein levels during 0-48 h of the wounding period. The transgenic lines exhibited appreciable levels of resistance against targeted insect pests in the leaf bioassays. The use of a wound-inducible promoter to drive insecticidal gene expression is a valuable insect resistant management strategy as gene expression will remain limited to the insect biting sites of plant and crop, food and environmental concerns can be minimized.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey) [2216]
dc.description.sponsorshipThe corresponding author is grateful to TUBITAK (The Scientific and Technological Research Council of Turkey) for providing postdoctoral fellowship Code: 2216) to carry out the research activities at University of Ankara. The authors acknowledge Dr. Umut Toprak, Associate Professor, Department of Plant Protection, Faculty of Agriculture, University of Ankara for providing Helicoverpa armigera for our lab bioassays experiment
dc.identifier.doi10.2298/ABS151012063B
dc.identifier.endpage780
dc.identifier.issn0354-4664
dc.identifier.issn1821-4339
dc.identifier.issue4
dc.identifier.scopus2-s2.0-85002942595
dc.identifier.scopusqualityQ3
dc.identifier.startpage773
dc.identifier.urihttps://dx.doi.org/10.2298/ABS151012063B
dc.identifier.urihttps://hdl.handle.net/11480/3759
dc.identifier.volume68
dc.identifier.wosWOS:000389771500008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherINST BIOLOSKA ISTRAZIVANJA SINISA STANKOVIC
dc.relation.ispartofARCHIVES OF BIOLOGICAL SCIENCES
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcotton modification
dc.subjectcry toxin
dc.subjectconfined expression
dc.subjectinsecticidal gene
dc.titleDEVELOPMENT OF INSECT-RESISTANT COTTON LINES WITH TARGETED EXPRESSION OF INSECTICIDAL GENE
dc.typeArticle

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