Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods

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dc.contributor.authorKaratepe B.
dc.contributor.authorAksoy S.
dc.contributor.authorKaratepe M.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2018
dc.departmentNiğde ÖHÜ
dc.description.abstractThe aim of this study was to determine the presence of Wolbachia spp. and Spiroplasma spp. in natural populations of sand flies in Turkey by molecular methods. A total of 40 Phlebotomus specimens (19 female and 21 male) were used in this study. Genomic DNA from whole sand flies was isolated and Wolbachia spp. infection prevalence was investigated by using Wolbachia gene specific primer sets (wsp and GroEL). In addition, the DNA were analyzed for the presence of Spiroplasma infections utilizing bacterium specific 16 S rDNA PCR-amplification primers. Results of this analysis showed a Wolbachia infection prevalence of 70% (28/40). There was no sex-bias in infection prevalence, being 76% (16/21) and 63% (12/19) in males and females, respectively. Analysis of Spiroplasma infections indicated that 26% (5/19) of female sand flies were positive for infection, while none of the screened males (0/21) were positive. Of the 40 sand fly samples, only 2 were found to be positive for both Wolbachia spp. and Spiroplasma spp. The present study demonstrates the presence of Wolbachia and Spiroplasma infections in the natural sand fly populations in Turkey. This is the first report on Spiroplasma infection in the sand flies from Turkey. © 2018 The Author(s).
dc.description.sponsorshipThis study was supported by TUBITAK (The Scientific and Technical Research Council of Turkey) (TUBITAK, BIDEB-2219). We are grateful to all the members of the Aksoy Lab for their contributions to the project especially Dr. Geoffrey M. Attardo for preparing tubulin primers and comments on the manuscript, Maria Gorreti Onyango, Michelle O’Neill and Yineng Yang for helping in Molecular Systematics Laboratory. We also thank Prof. Dr. Yusuf ÖZBEL for providing Phlebotomus samples that were collected for a previous TUBİTAK project (project number:112T270). We thank Mehmet Karakus from Aksoy laboratory for his critical reading of the paper. This study was presented at the XX. National Congress of Parasitology (25-29 September 2017, Eskişehir-Turkey).
dc.identifier.doi10.1038/s41598-018-29031-3
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.pmid30006543
dc.identifier.scopus2-s2.0-85049953330
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1038/s41598-018-29031-3
dc.identifier.urihttps://hdl.handle.net/11480/1579
dc.identifier.volume8
dc.identifier.wosWOS:000438489100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleInvestigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods
dc.typeArticle

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