A PROMISING AND COST EFFECTIVE SURFACE STERILIZING METHOD FOR SWEET POTATO (IPOMOEA BATATASE.) CULTIVATED IN OPEN ENVIRONMENT

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dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.contributor.authorBakhsh, Allah
dc.contributor.authorHussain, Tahira
dc.contributor.authorCaliskan, Mehmet Emin
dc.date.accessioned2024-11-07T13:24:28Z
dc.date.available2024-11-07T13:24:28Z
dc.date.issued2017
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractSweet potato is an important staple food and industrial material crop in the world. The crop is favored by resource poor farmers due to its good performance under adverse farming conditions, peak productivity in small farming areas and high carbohydrate and vitamin contents. The advances in genetic engineering technologies have helped the researchers to modify sweet potato to encode insect, herbicide and virus resistant traits. The efficient surface sterilization method is pre requisite for successful genetic improvement in sweet potato. We report an efficient, cost effective surface sterilization procedure of sweet potato seedlings collected from green house as well as field conditions for the initiation of in vitro culture. The seedlings from six sweet potato breeding lines were used to serve the purpose. Earlier, common bleach (NaOCl) was used to sterile sweet potato terminal buds to study their effectiveness in preventing microbial growth. Fungi and bacteria were most common microbial contaminants observed in cultures. Later on, the use of H2O2 in combination with ethanol resulted in relatively less contamination. The treatment of sweet potato ex plants with 70% ethanol for 5 min, 0.5% Mancozeb (Dithiocarbamate) for 5 min, followed by 5% hydrogen peroxide 5 mins resulted in suppressing microbial contaminations. This is the first report of the use of Mancozeb in combination with H2O2 for successful in vitro culture studies of sweet potato using explants from open environment. These findings will help to alleviate much burden associated with initiation of sweet potato cultures especially grown in field conditions.
dc.identifier.endpage3066
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue4
dc.identifier.startpage3061
dc.identifier.urihttps://hdl.handle.net/11480/14140
dc.identifier.volume26
dc.identifier.wosWOS:000400806100072
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectreproducible
dc.subjectsterilizing agents
dc.subjectefficiency
dc.subjectsweet potato
dc.titleA PROMISING AND COST EFFECTIVE SURFACE STERILIZING METHOD FOR SWEET POTATO (IPOMOEA BATATASE.) CULTIVATED IN OPEN ENVIRONMENT
dc.typeArticle

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