Immobilization of tyrosinase and alcohol oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole

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dc.authorid0000-0002-7324-0385
dc.contributor.authorYildiz, Huseyin Bekir
dc.contributor.authorSahmetlioglu, Ertugrul
dc.contributor.authorBoyukbayram, Ayse Elif
dc.contributor.authorToppare, Levent
dc.contributor.authorYagci, Yusuf
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2007
dc.departmentNiğde ÖHÜ
dc.description.abstractImmobilization of tyrosinase and alcohol oxidase is achieved in the copolymer of pyrrole with vinyl alcohol with thiophene side groups (PVATh-co-PPy) which is a newly synthesized conducting polymer. PVATh-co-PPy/alcohol oxidase and PVATh-co-PPy/tyrosinase electrodes are constructed by the entrapment of enzyme in conducting copolymer matrix during electrochemical copolymerization. For tyrosinase and alcohol oxidase enzymes, catechol and ethanol are used as the substrates, respectively. Kinetic parameters: maximum reaction rates (V-max) and Michaelis-Menten constants (K-m) are obtained. V-max and K-m are found as 2.75 mu mol/(min electrode) and 18 mM, respectively, for PVATh-coPPy/alcohol oxidase electrode and as 0.0091 mu mol/(min electrode) and 40 mM, respectively, for PVATh-co-PPy/tyrosinase electrode. Maximum temperature and pH values are investigated and found that both electrodes have a wide working range with respect to both temperature and pH. Operational and storage stabilities show that although they have limited storage stabilities, the enzyme electrodes are useful with respect to operational stabilities. (c) 2007 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.ijbiomac.2007.04.006
dc.identifier.endpage337
dc.identifier.issn0141-8130
dc.identifier.issue3
dc.identifier.pmid17555810
dc.identifier.scopus2-s2.0-34250867337
dc.identifier.scopusqualityQ1
dc.identifier.startpage332
dc.identifier.urihttps://dx.doi.org/10.1016/j.ijbiomac.2007.04.006
dc.identifier.urihttps://hdl.handle.net/11480/5362
dc.identifier.volume41
dc.identifier.wosWOS:000248355400016
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectconducting polymer
dc.subjectelectrochemical polymerization
dc.subjectsensors
dc.subjectenzymes
dc.subjectimmobilization
dc.subjectalcohol oxidase
dc.subjecttyrosinase
dc.subjectethanol
dc.subjectpolyphenols
dc.titleImmobilization of tyrosinase and alcohol oxidase in conducting copolymers of thiophene functionalized poly(vinyl alcohol) with pyrrole
dc.typeArticle

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