Denitrification performance and microbial community dynamics in a denitrification reactor as revealed by high-throughput sequencing
Küçük Resim Yok
Tarih
2017
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Iwa Publishing
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The dynamics of the bacterial community associated with the denitrification process in a fixed bed column reactor (FBCR) were investigated using 454-pyrosequencing methodology. A FBCR filled with elemental sulfur and limestone was operated for about 94 days under autotrophic and mixotrophic (autotrophic + heterotrophic) conditions at 30 degrees C. Efficient simultaneous bromate and nitrate removal was achieved at feed concentrations of 500 mu g/L bromate and 45 mg/L nitrate under autotrophic and mixotrophic conditions. Operational taxonomic units-based analysis (97% similarity cut-off) of bioreactor samples (three periods) revealed that the microbial diversity changed regardless of operational conditions. Sulfurimonas spp. was dominant in the reactor at the adaptation stage. Thiobacillus denitrificans is a chemolithoautotrophic bacterium that is capable of the oxidation of inorganic sulfur compounds. After the adaptation period, the microbial profile changed such that Spirochaetacea spp. and Denitratisoma spp. were major species in the column reactor. After 60 d of operation, Hyphomicrobium vulgare became dominant due to the mixotrophic denitrification conditions.
Açıklama
Anahtar Kelimeler
bromate, high-throughput sequencing, microbial community, mixotrophic denitrification, nitrate
Kaynak
Water Science and Technology-Water Supply
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
17
Sayı
4
