Characterization of ATPases in the gill of freshwater mussel (Unio tigridis) and effects of ionic and nanoparticle forms of aluminium and copper

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dc.authorid/0000-0002-0132-3712
dc.authoridCanli, Mustafa/0000-0002-7038-6186
dc.contributor.authorCanli, Esin G.
dc.contributor.authorCanli, Mustafa
dc.date.accessioned2024-11-07T13:31:23Z
dc.date.available2024-11-07T13:31:23Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractMussels are often used to monitor the aquatic systems for different ecological aims, as they are one of the best bioindicator animals. However, the characterization of biomarkers should be known before using them in environmental monitoring and toxicology studies. There is no study to our knowledge on the characterization of Na-ATPase and Ca-ATPase in tissues of freshwater mussel (Unio tigridis). Thus, this study was undertaken to characterize the optimum working conditions of Na-ATPase and Ca-ATPase in the gill of mussels, determining the highest levels of parameters (Na+, Mg2+, Ca2+, ATP, pH, temperature, enzyme amount, incubation time) to obtain maximum activity. The present study also aimed to investigate in vitro effects of ionic and nanoparticle (Al2O3, CuO) forms of aluminium and copper (0, 30, 90, 270 mu g/L) on the activities of Na-ATPase and Ca-ATPase. Data showed that there was no ouabain-sensitive ATPase activity in the gill up to 10 mM ouabain concentrations. Na-ATPase and Ca-ATPase activities in the gill of control mussels were 5.124 +/- 0.373 and 3.750 +/- 0.211 mu mol Pi/mg pro./h, respectively. Exposure to different concentrations of nanoparticles did not alter significantly (P 0.05) the activities of Na-ATPase and Ca-ATPase in vitro, whereas the same concentrations of ionic aluminium and copper significantly decreased (P < 0.05) the enzyme activities. Data emphasized that there were different modes of action between ionic and nanoparticle forms of aluminium and copper. Data also suggested that in vivo studies should also be carried out to estimate better the effects of nanoparticle and ionic forms of metals on ATPases of U. tigridis.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (Tubitak) [120Y016]
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey (Tubitak, 120Y016).
dc.identifier.doi10.1016/j.cbpc.2021.109059
dc.identifier.issn1532-0456
dc.identifier.issn1878-1659
dc.identifier.pmid33901633
dc.identifier.scopus2-s2.0-85105358785
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.cbpc.2021.109059
dc.identifier.urihttps://hdl.handle.net/11480/14816
dc.identifier.volume247
dc.identifier.wosWOS:000671438100002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Inc
dc.relation.ispartofComparative Biochemistry and Physiology C-Toxicology & Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectCharacterization
dc.subjectATPase
dc.subjectMussel
dc.subjectAluminium
dc.subjectCopper
dc.subjectNanoparticle
dc.titleCharacterization of ATPases in the gill of freshwater mussel (Unio tigridis) and effects of ionic and nanoparticle forms of aluminium and copper
dc.typeArticle

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