Punicic Acid Inhibits Glioblastoma Migration and Proliferation via the PI3K/AKT1/mTOR Signaling Pathway

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dc.authoridunsal, ulkun unlu/0000-0001-5194-3138
dc.authoridAYDEMIR, ISIL/0000-0002-4143-7319
dc.contributor.authorMete, Mesut
dc.contributor.authorUnsal, Ulkun U.
dc.contributor.authorAydemir, Isil
dc.contributor.authorSonmez, Pinar K.
dc.contributor.authorTuglu, Mehmet, I
dc.date.accessioned2024-11-07T13:34:29Z
dc.date.available2024-11-07T13:34:29Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground: Punicic Acid (PA) is a polyunsaturated fatty acid that accounts for approximately 70%-80% of Pomegranate Seed Oil (PSO). PA possesses strong antioxidant, anti-inflammatory, anti-atherogenic effects, and anti-tumorigenic properties. Pomegranate extracts have been shown to have anticancer activity in many studies. However, there is no evidence for the effect of PSO on T98 glioblastoma cells. Therefore, the present study was the first to investigate the mechanisms induced by PA on T98 cells, which is one of the major compounds extracted from PSO. Methods: The effects of PA on cell viability; oxidative stress; and migration, proliferation, and apoptosis at the IC50 close were studied. Results: The proliferation and migration were inhibited in the treated group compared to the non-treated group by 9.85 mu l/ml PA. The difference was statistically significant (***p<0.001). Furthermore, PA-induced apoptosis in the T98 glioblastoma cells compared to non-treated group and the difference was statistically significant (***p<0.001). Apoptosis was determined via immunocytochemistry staining of caspase-3, caspase-9 and TUNEL methods. Apoptosis was checked by flow cytometry (using caspase 3 methods) and Scanning Electron Microscopy Analysis. We also investigated the potential signaling pathway underlying this apoptotic effect. The immunocytochemical stainings of PI3K/ Akt-1/ mTOR-1 demonstrated that Akt-1 staining was increased with PA treatment similar to mTOR-1 and PI3K staining (***p<0.001). These increases were statistically significant compared to the non-treated group. Conclusion: PA exhibited exceptional abilities as an anticancer agent against GBM cells. The use of punicic acid in combination with other drugs used in the treatment of glioblastoma may increase the efficacy of the treatment. This study provided a basis for future investigation of its use in preclinical and clinical studies.
dc.description.sponsorshipTurkish Neurosurgical Society
dc.description.sponsorshipPreparation for publication of this article was partly supported by the Turkish Neurosurgical Society.
dc.identifier.doi10.2174/1871520619666190405112507
dc.identifier.endpage1131
dc.identifier.issn1871-5206
dc.identifier.issn1875-5992
dc.identifier.issue9
dc.identifier.pmid30950355
dc.identifier.scopus2-s2.0-85074962565
dc.identifier.scopusqualityQ3
dc.identifier.startpage1120
dc.identifier.urihttps://doi.org/10.2174/1871520619666190405112507
dc.identifier.urihttps://hdl.handle.net/11480/16007
dc.identifier.volume19
dc.identifier.wosWOS:000492373400004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofAnti-Cancer Agents in Medicinal Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectPunicic acid
dc.subjectglioblastoma
dc.subjectPI3K/AKT1/mTOR
dc.subjectsignaling pathway
dc.subjectglioblastoma multiforme
dc.subjecttumor infiltration
dc.subjectapoptosis
dc.titlePunicic Acid Inhibits Glioblastoma Migration and Proliferation via the PI3K/AKT1/mTOR Signaling Pathway
dc.typeArticle

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