Molecular Identification of Fig Cryptic Virus and Fig Fleck-Associated Virus in Turkey

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dc.authoridElci, Eminur/0000-0002-6434-6321
dc.contributor.authorElci, Eminur
dc.contributor.authorHancer, Tugce
dc.contributor.authorCaglayan, Kadriye
dc.date.accessioned2024-11-07T13:31:52Z
dc.date.available2024-11-07T13:31:52Z
dc.date.issued2017
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractRecently, several new viruses infecting fig trees were identified. To assess the presence, distribution and genetic diversity of Fig cryptic virus (FCV) and Fig fleck-associated virus (FFkaV) in fig trees of Turkey, a total of 65 fig samples, which show yellowing, chlorotic, necrotic spots and vein clearing symptoms, were collected from Aegean and Mediterranean regions, which are the most important fig growing regions of Turkey, in spring 2014 and tested by molecular analysis. After cDNA synthesis, FCV and FFkaV specific primer sets of RNA dependent RNA polymerase (RdRp) regions were used for reverse transcription-polymerase chain reaction (RT-PCR) analysis and the PCR products were directly sequenced. The obtained sequences were analyzed and nucleotide sequence analysis confirmed the FCV and FFkaV infections. According to the results, some of the fig trees are infected in Turkey by FCV and FFkaV with an incidence of 20 % and 9.2 %, respectively. BLAST analysis of both FCV and FFkaV has shown high identity with Italian isolates (FCV\ref\NC015494.1 and FFkaV\ref\NC015229.1). For FFkaV, the phylogenetic analysis, constructed from partial RdRp nucleotide sequences, clustered the isolates based on their geographical origin. While the correlation between FFkaV isolates and regions was very high, no correlation between collection regions and FCV isolates was observed. It can be concluded that, fig trees from the most important fig growing regions of Turkey are infected by FCV and FFkaV and it is instrumental to overview of the viral control strategies for fig plantations in Turkey.
dc.identifier.endpage12390
dc.identifier.issn1224-5984
dc.identifier.issue2
dc.identifier.startpage12383
dc.identifier.urihttps://hdl.handle.net/11480/15088
dc.identifier.volume22
dc.identifier.wosWOS:000403059300009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherArs Docendi
dc.relation.ispartofRomanian Biotechnological Letters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectFicus carica L.
dc.subjectFCV
dc.subjectFFkaV
dc.subjectRT-PCR
dc.subjectRdRp
dc.subjectsequencing
dc.titleMolecular Identification of Fig Cryptic Virus and Fig Fleck-Associated Virus in Turkey
dc.typeArticle

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