Effects of thymoquinone in prevention of experimental contrast-induced nephropathy in rats

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dc.authoridYAZICI, Cevat/0000-0003-0625-9542
dc.authoridDogan, Muhammet Ensar/0000-0002-5034-7083
dc.authoridELMALI, FERHAN/0000-0002-1967-1811
dc.authoridGunturk, Inayet/0000-0002-8299-1359
dc.contributor.authorTopaloglu, Ulan Serkan
dc.contributor.authorSipahioglu, Murat Hayri
dc.contributor.authorGunturk, Inayet
dc.contributor.authorAkgun, Hulya
dc.contributor.authorDogan, Muhammet Ensar
dc.contributor.authorSonmez, Gokhan
dc.contributor.authorElmali, Ferhan
dc.date.accessioned2024-11-07T13:34:04Z
dc.date.available2024-11-07T13:34:04Z
dc.date.issued2019
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractObjective(s): This study aimed to show the effects of thymoquinone, which is known for its antioxidant, anti-inflammatory, and renal protective effects in contrast-induced nephropathy. Materials and Methods: This is an experimental study in rats. 7 groups were included within the scope of our study: sham-vehicle (n=3), premedication-control (n=6), model (n=6), isolated thymoquinone (n=3+3), low-dose thymoquinone (n=6), and high-dose thymoquinone (n=7). In addition to 48 hr of water deprivation, we pre-medicated the rats with intra-peritoneal indomethacin and L-NAME administration. After premedication, 12.5 ml/kg dose of a high osmolar contrast agent-diatrizoat (Urografin %76) was administrated. Thymoquinone was administrated in two different doses of 1 mg/kg and 1.75 mg/kg for four days intraperitoneally. Renal functions, histopathological differences, oxidative stress parameters, and inflammatory indicators of rats were evaluated at the end of the study. Results: Significant decreases were observed in levels of serum creatinine and serum BUN with low-dose thymoquinone (1 mg/kg) administration. In light microscopy, significantly less histopathological damage was observed in the low-dose thymoquinone group compared to the contrast agent group. While high-dose thymoquinone is accepted as ineffective biochemically, toxic evidence was identified histopathologically. There were no significant differences between M and TA groups for serum MDA and SOD levels, which were compared to evaluate oxidative stress (P:0.99, P:0.98; respectively). TNF-alpha, iNOS, and NF-kappa B gene expressions were not significantly different between all groups (P:0.748, P:0.531, P:0.910; respectively). Conclusion: This experimental study has demonstrated for the first time the protective effect of the TQ substance for CIN in 1 mg/kg dose, in the accompaniment of biochemical and histopathological data in rats.
dc.description.sponsorshipErciyes University [TTU-2014-5096]
dc.description.sponsorshipThe results presented in this paper were part of a student thesis. This work was supported by Research Fund of the Erciyes University (project number: TTU-2014-5096). Informed consent needed for the study was received from Erciyes University Experimental Animals Ethical Committee (date: 09.04.2014, decision no: 14/74). We extend our thanks to Cagri Sakalar for his contribution to experiments.
dc.identifier.doi10.22038/IJBMS.2019.13990
dc.identifier.endpage1439
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.issue12
dc.identifier.pmid32133061
dc.identifier.startpage1432
dc.identifier.urihttps://doi.org/10.22038/IJBMS.2019.13990
dc.identifier.urihttps://hdl.handle.net/11480/15775
dc.identifier.volume22
dc.identifier.wosWOS:000491224300009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMashhad Univ Med Sciences
dc.relation.ispartofIranian Journal of Basic Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectContrast-induced-nephropathy
dc.subjectInflammation
dc.subjectOxidative stress
dc.subjectNigella sativa
dc.subjectRat
dc.subjectThymoquinone
dc.titleEffects of thymoquinone in prevention of experimental contrast-induced nephropathy in rats
dc.typeArticle

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