Effect of thymoquinone on NRF2/NF-kB/MAPK pathway in methotrexate-induced rat testis injury

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dc.authoridCEYLAN, TAYFUN/0000-0002-0917-0378
dc.contributor.authorKaymak, Emin
dc.contributor.authorCeylan, Tayfun
dc.contributor.authorAkin, Tugrul
dc.contributor.authorKuloglu, Nurhan
dc.contributor.authorSayan, Meryem
dc.contributor.authorDegen, Necla
dc.contributor.authorOnal, Esra
dc.date.accessioned2024-11-07T13:35:02Z
dc.date.available2024-11-07T13:35:02Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractObjective(s): In this study, we aimed to investigate the protective effect of Thymoquinone (THQ) against testicular damage caused by Methotrexate (MTX). Materials and Methods: This study consists of 5 groups: Control, Olive oil, THQ, MTX, and MTX+THQ. At the end of the experiment, spermiogram analysis was performed on the rats. In addition, testicular tissues were taken and histopathology, immunohistochemistry, and biochemistry analysis were performed. Biochemical analyses were performed on the serums. Results: According to the results obtained, spermiogram values, Johnson's testicular biopsy score, SOD, CAT, GPx, FSH, LH, and testosterone values were statistically significantly decreased in the MTX group compared to the control group. In the MTX+THQ group, spermiogram values, Johnson's testicular biopsy score, SOD, CAT, GPx, FSH, LH, and testosterone values increased statistically significantly compared to the MTX group. NRF2 and HO-1 immunoreactivity were statistically significantly decreased in the MTX group compared to the control group. In the MTX+THQ group, NRF2 and HO-1 immunoreactivity were statistically significantly increased compared to the MTX group. The level of MDA, which is important in lipid damage, and the level of biochemistry results of TNF-alpha, IL1-beta, and IL-6, which are important markers, and the results of p-NF-kB and P38 immunoreactivity were statistically significantly increased in the MTX group compared to the control group. In the MTX+THQ group, these parameters showed a significant decrease compared to the MTX group. Conclusion: According to these results, it is thought that THQ will play a protective role against infertility caused by chemotherapy-induced testicular damage.
dc.description.sponsorshipYozgat Bozok Universimath;ty Scientific Research Projects Coordination Unit [6602c-TF/21-458]
dc.description.sponsorshipThis Project [Project No: 6602c-TF/21-458] Was Supported By the Yozgat Bozok Univers & imath;ty Scientific Research Projects Coordination Unit.
dc.identifier.doi10.22038/ijbms.2024.77714.16813
dc.identifier.issn2008-3866
dc.identifier.issn2008-3874
dc.identifier.issue11
dc.identifier.pmid39386231
dc.identifier.scopus2-s2.0-85204090007
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.22038/ijbms.2024.77714.16813
dc.identifier.urihttps://hdl.handle.net/11480/16303
dc.identifier.volume27
dc.identifier.wosWOS:001317362700008
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherMashhad Univ Med Sciences
dc.relation.ispartofIranian Journal of Basic Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectInflammation
dc.subjectMethotrexate
dc.subjectOxidative stress
dc.subjectTestis
dc.subjectThymoquinone
dc.subjectIntroduction
dc.titleEffect of thymoquinone on NRF2/NF-kB/MAPK pathway in methotrexate-induced rat testis injury
dc.typeArticle

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