Investigating effect of miR160 through overexpression in potato cultivars under single or combination of heat and drought stresses

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dc.authoridOZTURK, ZAHIDE NESLIHAN/0000-0002-2592-9997
dc.contributor.authorSanli, Beyazit Abdurrahman
dc.contributor.authorOzturk Gokce, Zahide Neslihan
dc.date.accessioned2024-11-07T13:24:15Z
dc.date.available2024-11-07T13:24:15Z
dc.date.issued2021
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPotato (Solanum tuberosum L.) is one of the major tuber crops which is widely grown in the world. Potato growth and development as well as yield is negatively affected by heat and drought stress alone, but combined stress has devastating effect. MicroRNAs are group of small RNAs which play vital role in gene regulation at transcriptional and post-transcriptional level by repressing or inhibiting protein expression of corresponding target. Current study aimed to understand the function of miR160a-5p and its influence on its target gene ARF16 under applied stresses. Potato cultivars tolerant (Unica) and sensitive (Russet Burbank) were tested under drought, heat, and their combination of stresses. The expression of miR160a-5p was revealed to be higher along with the suppression of corresponding target gene ARF16 gene. Transgenic plants also showed improved physiological, and biochemical traits, with lower accumulation of H2O2 and higher levels of proline solely under drought stress conditions. It was also observed that overexpression of miR160a-5p could alter mechanisms for auxin response factor pathways which enabled transgenic plants to have higher tolerance to abiotic stress conditions. Based on our findings, transgenic Unica and Russet Burbank plants showed increased tolerance to drought and heat stress as compared to wild-type plants. This study can be helpful in understanding the role of miR160a-5p in improved tolerance to abiotic stresses.
dc.description.sponsorshipTUBITAK [115o405]
dc.description.sponsorshipThis study was supported by TUBITAK (Project number 115o405). We would like to acknowledge project scholars (Arslan Asim, Melis Yalcn, Esra Kaplan, Hussain Azimi, lknur Tnda) and members (Prof. Dr. Sevgi Calkan, Prof. Dr. Mehmet Emin Calkan, Assoc. Prof. Dr. Ufuk Demirel, Dr. Allah Bakhsh and Dr. Emre Aksoy) for their contribution. We also would like to acknowledge Dr. Usman Khalid Chaudhry and Muhammad Daniyal Junaid for their support in the preparation of this manuscript.
dc.identifier.doi10.1007/s11816-021-00677-2
dc.identifier.endpage348
dc.identifier.issn1863-5466
dc.identifier.issn1863-5474
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85106043733
dc.identifier.scopusqualityQ2
dc.identifier.startpage335
dc.identifier.urihttps://doi.org/10.1007/s11816-021-00677-2
dc.identifier.urihttps://hdl.handle.net/11480/14016
dc.identifier.volume15
dc.identifier.wosWOS:000651377600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPlant Biotechnology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectPotato
dc.subjectDrought stress
dc.subjectHeat stress
dc.subjectmiRNA
dc.subjectmiR160
dc.subjectTransgenic
dc.titleInvestigating effect of miR160 through overexpression in potato cultivars under single or combination of heat and drought stresses
dc.typeArticle

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