Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

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dc.authoridBakhsh, Allah/0000-0003-3561-7863
dc.authoridHossain, Md Jakir/0000-0003-1076-4867
dc.contributor.authorHossain, Md. Jakir
dc.contributor.authorBakhsh, Allah
dc.contributor.authorJoyia, Faiz Ahmad
dc.contributor.authorAksoy, Emre
dc.contributor.authorGokce, Neslihan Zahide Ozturk
dc.contributor.authorKhan, Muhammad Sarwar
dc.date.accessioned2024-11-07T13:35:04Z
dc.date.available2024-11-07T13:35:04Z
dc.date.issued2023
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0 mg/l), NAA (0.2 mg/l), TDZ (2.0 mg/l) and GA3 (0.1 mg/l); spectinomycin 50 mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85.7%) of transplastomic shoots. Sante and Challenger showed 9.6% shoot regeneration efficiency followed by cultivar Simply Red (8.8%). PCR amplification yielded 16 postive transplastomic plantlets out of 21 spectinomycin resistant ones. Target gene integration was confirmed by PCR and Southern blot, whereas RT-qPCR was used to assess the expression level of transgene. The localization of visual marker gene gfp was tracked by laser scanning confocal microscopy which confirmed its expression in chloroplasts of leaf cells. The transplastomic plants ensured high mortality to both larvae and adult CPB. Foliage consumption and weight gain of CPB fed on transplastomic leaves were lower compared to the control plants. Sucessful implementation of current research findings can lead to a viable solution to CPB mediated potato losses globally.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [216O027]; Pakistan Science Foundation (PSF); Tubitak-PSF
dc.description.sponsorshipThis research is part of the PhD thesis of Md Jakir Hossain, who completed research work out of project funded by the Scientific and Technological Research Council of Turkey (Tubitak, Project 216O027), Turkey and Pakistan Science Foundation (PSF). We highly acknowledge Tubitak-PSF for financial support to conduct our current research.
dc.identifier.doi10.1007/s11248-023-00366-6
dc.identifier.endpage512
dc.identifier.issn0962-8819
dc.identifier.issn1573-9368
dc.identifier.issue6
dc.identifier.pmid37707659
dc.identifier.scopus2-s2.0-85171266393
dc.identifier.scopusqualityQ2
dc.identifier.startpage497
dc.identifier.urihttps://doi.org/10.1007/s11248-023-00366-6
dc.identifier.urihttps://hdl.handle.net/11480/16321
dc.identifier.volume32
dc.identifier.wosWOS:001068058700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofTransgenic Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241106
dc.subjectCPB
dc.subjectInsect resistance
dc.subjectBacillus thuringiensis
dc.subjectTransplastomic approach
dc.titleEngineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)
dc.typeArticle

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