Comparison of expression patterns of selected drought-responsive genes in barley (Hordeum vulgare L.) under shock-dehydration and slow drought treatments

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dc.authorid0000-0002-9526-576X
dc.contributor.authorGurel, Filiz
dc.contributor.authorOzturk, Neslihan Z.
dc.contributor.authorYoruk, Emre
dc.contributor.authorUcarli, Cuneyt
dc.contributor.authorPoyraz, Nazaret
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.departmentNiğde ÖHÜ
dc.description.abstractShock-like water stress using hydroponics and gradual water deficit in soil are the two widely used treatments to analyze transcriptional response of many crops to drought. In this study, we investigated the effects of shock drought (ShD) (0, 0.5, 1, 4, and 8 h) and slow drought (SDD) [soil water content (SWC) of 35 and 50 %] on the expression of well-known drought-responsive genes supplemented with physiological changes in barley. Two barley cultivars with contrasting leaf relative water content (RWC) and water loss rate (WLR) values were selected as Marti (MR; 60 % RWC and 0.046 gh(-1) g(-1) DW) and Erginel90 (ER; 38.3 % RWC and 0.350 gh(-1) g(-1) DW) under 38 % of SWC condition. According to the results, 0.5 h ShD was the critical time point for stress perception in leaves defined by the increase in WLR, ion leakage and H2O2 concentration. Expressions of antioxidant-related genes (Cu-Zn/SOD, HvCAT2, HvGST6, HvAPX) were rapidly induced in MR at 8 h shock, while only slightly upregulated in ER. We have also observed higher induction of expressions of HvBAS1, HvMT-2, HvABA7 and a photosynthesis-related gene HvLHCB during ShD compared to SDD. Contrarily, transcription factors (TFs), HvWRKY12 and HvDRF1 were expressed with lower values during shock-dehydration. Slow-drought treatments in both cultivars were characterized with high leaf RWCs and osmotic adjustment with low cell membrane damage, suggesting that barley maintains a basal tolerance to long-term water deficit. Our results confirmed that type of water stress treatment is crucial to measure gene expression, and a shock-like dehydration method should be the treatment of choice in evaluating barley plants with different physiological characteristics for water tolerance.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul University [BAP 4712]
dc.description.sponsorshipThis work was supported by The Scientific Research Projects Coordination Unit of Istanbul University, Project Number BAP 4712. The authors thank to the staff of Dr. Maria von Korff's lab for experimental advice on stress applications and qRT-PCR.
dc.identifier.doi10.1007/s10725-016-0156-0
dc.identifier.endpage193
dc.identifier.issn0167-6903
dc.identifier.issn1573-5087
dc.identifier.issue2
dc.identifier.scopus2-s2.0-84959330543
dc.identifier.scopusqualityQ1
dc.identifier.startpage183
dc.identifier.urihttps://dx.doi.org/10.1007/s10725-016-0156-0
dc.identifier.urihttps://hdl.handle.net/11480/3550
dc.identifier.volume80
dc.identifier.wosWOS:000385480800008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherSPRINGER
dc.relation.ispartofPLANT GROWTH REGULATION
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBarley
dc.subjectDrought
dc.subjectStress related gene expression
dc.subjectqRT-PCR
dc.subjectH2O2
dc.titleComparison of expression patterns of selected drought-responsive genes in barley (Hordeum vulgare L.) under shock-dehydration and slow drought treatments
dc.typeArticle

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