Towards multimodal HPLC separations on humic acid-bonded aminopropyl silica: RPLC and LEC behavior

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dc.authorid0000-0001-6808-2282
dc.contributor.authorGezici, Orhan
dc.contributor.authorKara, Huseyin
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2011
dc.departmentNiğde ÖHÜ
dc.description.abstractIn the present study, metal binding property of humic acid (HA) was successfully adapted to the ligand-exchange concept, and metal-loaded immobilized humic acid was used as a ligand exchanger stationary phase for separation of some nucleosides. Humic-acid bonded aminopropyl silica (EC-HA-APS) was turned into ligand exchanger forms by loading aqueous solutions of Cu(2+), and Co(2+) to the column (4.6 X 150; as mm) packed with EC-HA-APS. Metal ion solutions were loaded to the column in a step-wise manner where the concentration of metal ion solution being loaded to the column was increased gradually between 5 and 100 mM. The progress of metal loading process was monitored via the breakthrough curves propagated stepwise. Ligand-exchange chromatography (LEC) studies were performed on an HPLC system, and chromatographic behaviors of the studied nucleosides (i.e. uridine, Urd; thymidine, Tyd; cytidine, Cyd; adenosine, Ado; and guanosine, Guo) were investigated on Cu(2+) and Co(2+) loaded forms of the EC-HA-APS (Cu-EC-HA-APS and Co-EC-HA-APS). Effect of mobile phase composition, temperature, and the type of metal ion loaded to the column on the retentive behaviors of the compounds was studied, in detail. The studied solutes exhibited mixed-mode RPLC/LEC behavior on the stationary phase. Metal-loaded column (M-EC-HA-APS) was easily regenerated into its original form. EC-HA-APS, with 98 +/- 2% metal recoveries, by using aqueous mixture of EDTA + NH(3) at pH = 7.5. Thus, the stationary phase exhibited a high flexibility between RPLC and LEC modes. This property, also, made it possible to convert the stationary phase into various ligand exchanger forms by loading different metal ions. Hence, capacity and selectivity of the stationary phase towards the studied species was manipulated easily by loading different metal ions to the stationary phase. Baseline separation for the studied species was achieved on Cu-EC-HA-APS and Co-EC-HA-APS and some differentiations were observed in capacity and selectivity, depending on the type of metal loaded. Thus, being as the first endeavor on usability of immobilized HA as a ligand exchanger stationary phase, the present study is believed to be useful to understand multifunctional character of HA-based solid/stationary phases. (C) 2011 Elsevier B.V. All rights reserved.
dc.description.sponsorshipSelcuk University BAP [082011043]
dc.description.sponsorshipAuthors gratefully acknowledge the financial support provided by Selcuk University BAP. Project Number: 082011043,
dc.identifier.doi10.1016/j.talanta.2011.07.076
dc.identifier.endpage2410
dc.identifier.issn0039-9140
dc.identifier.issue5
dc.identifier.pmid21962660
dc.identifier.scopus2-s2.0-80053563013
dc.identifier.scopusqualityQ1
dc.identifier.startpage2405
dc.identifier.urihttps://dx.doi.org/10.1016/j.talanta.2011.07.076
dc.identifier.urihttps://hdl.handle.net/11480/4680
dc.identifier.volume85
dc.identifier.wosWOS:000296115600021
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.relation.ispartofTALANTA
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectHumic acid
dc.subjectImmobilization
dc.subjectLigand-exchange chromatography
dc.subjectNucleosides
dc.subjectSeparation
dc.subjectStationary phase
dc.titleTowards multimodal HPLC separations on humic acid-bonded aminopropyl silica: RPLC and LEC behavior
dc.typeArticle

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